Distinct reactivities of four monoclonal antibodies with human interleukin 2 receptor.

Abstract

Two new murine monoclonal IgG1 antibodies, H-31 and H-A26, were characterized in comparison with two previously obtained monoclonal antibodies against human interleukin 2 (IL-2) receptor (IL-2 R), anti-Tac and HIEI. In immunofluorescence assays with various human hematopoietic cells, H-31 and H-A26 antibodies both reacted with only IL-2 R-positive cells, and they precipitated IL-2 R molecules, glycoproteins with molecular weights of 60K and 53K daltons (gp60/gp53), from human T-cell leukemia virus type I (HTLV-I)-carrying MT-2 cells, as demonstrated by sequential immunoprecipitation after absorption of IL-2 R with anti-Tac. Antibody-binding competition assays showed that H-31 and anti-Tac, and H-A26 and HIEI, respectively, competed reciprocally in binding to the cells, and that anti-Tac also inhibited the binding of HIEI but not vice versa. H-31, like anti-Tac, strongly inhibited the IL-2-dependent proliferation of normal activated T-cells, absorption of IL-2 and direct binding of IL-2 to the cells, while H-A26, like HIEI, inhibited those processes only weakly. The spectra of reactivities of these antibodies with various simian cell lines derived by HTLV-I infection were different, as revealed by immunofluorescence studies. Human IL-2 R was shown to express a unique antigenic determinant, detected with HIEI, that was not detectable in IL-2 R molecules of Old and New World monkeys, and also to express determinants common to simian IL-2 R molecules. These observations indicate that H-31 and H-A26 recognize human IL-2 R molecules and that the antigenic sites on the IL-2 R molecule defined by H-31, H-A26, anti-Tac, and HIEI are different.

• Authors: Y Tanaka, H Tozawa, M Hayami, K Sugamura, Y Hinuma
• Journal: Microbiology and immunology (Microbiol Immunol) Vol. 29 Issue 10 Pg. 959-72 ( 1985) ISSN: 0385-5600 [Print] Australia
• PMID: 2417094 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, Surface
• Epitopes
• Phytohemagglutinins
• Receptors, Immunologic
• Receptors, Interleukin-2
• Tumor Necrosis Factor Receptor Superfamily, Member 7

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antibody Specificity
• Antigens, Surface (immunology)
• Binding, Competitive
• Cell Transformation, Viral
• Deltaretrovirus
• Epitopes (immunology)
• Haplorhini
• Humans
• Lymphocyte Activation
• Mice
• Phytohemagglutinins (pharmacology)
• Receptors, Immunologic (immunology)
• Receptors, Interleukin-2
• Species Specificity
• T-Lymphocytes (immunology)
• Tumor Necrosis Factor Receptor Superfamily, Member 7