Protective effect of infliximab on ischemia/reperfusion injury in a rat ovary model: biochemical and histopathologic evaluation.

Abstract

OBJECTIVE:

The aim of this study was to investigate the effect of infliximab on experimentally induced ovarian ischemia/reperfusion injury (IRi).

STUDY DESIGN:

A total of 42 female rats were equally divided into 6 experimental groups; group 1: sham operation, group 2: 3-h ischemia, group 3 and 4: 3-h ischemia, 3-h reperfusion, group 5 and 6: 3-h ischemia, 24h reperfusion. In group 4 and group 6, 30 min before reperfusion, infliximab was administered intraperitoneally at a dose of 5mg/kg. Bilateral ovaries were removed for histopathologic and biochemical analysis. Serum MDA (sMDA), tissue MDA (tMDA), serum NO (sNO), tissue NO (tNO) and serum catalase concentrations were analyzed. Tissue damage of ovarian tissue was scored by histological examination.

RESULTS:

The infliximab administration significantly lowered the sNO, tNO and sMDA concentrations in group 4 compared to group 3 (p=0.041, p=0.025 and p=0.035, respectively). sNO, tNO and sMDA concentrations were also lower in group 6 when compared to group 5, but this differences were not significant (p>0.05). On the other hand, tMDA concentrations were lower in infliximab-applied groups when compared to ischemia/reperfusion groups (group 3 vs. 4 and 5 vs. 6) (p=0.045 and p=0.048, respectively). Moreover, histopathologic tissue damage scores in infliximab administration groups were significantly lower than in ischemia/reperfusion groups (p<0.001).

CONCLUSION:

Infliximab attenuates I/R-induced ovarian tissue injury in rats subjected to ischemia/reperfusion.

• Authors: Remzi Abali, Nicel Tasdemir, Mehmet Aytac Yuksel, Savas Guzel, Meltem Oznur, Burcin Nalbantoglu, Ufuk Goker Tasdemir
• Journal: European journal of obstetrics, gynecology, and reproductive biology (Eur J Obstet Gynecol Reprod Biol) Vol. 171 Issue 2 Pg. 353-7 (Dec 2013) ISSN: 1872-7654 [Electronic] Ireland
• PMID: 24169036 (Publication Type: Journal Article)
• Copyright: Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Antibodies, Monoclonal
• Antioxidants
• Nitric Oxide
• Malondialdehyde
• Infliximab
• Catalase

Topics

• Animals
• Antibodies, Monoclonal (therapeutic use)
• Antioxidants (therapeutic use)
• Catalase (blood)
• Female
• Infliximab
• Malondialdehyde (metabolism)
• Nitric Oxide (metabolism)
• Ovary (injuries)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (drug therapy, pathology, prevention & control)
• Torsion Abnormality (therapy)