The utility of the BIOMED-2 primers in the detection of 2 clonal, B-lymphoproliferative disorders simultaneously involving the same site.

Abstract

CONTEXT:

Molecular tests for clonality performed on atypical lymphoid lesions may yield abnormal results because of the coexistence of monoclonal B lymphocytosis or monoclonal gammopathy of undetermined significance in the sample investigated.

OBJECTIVE:

To investigate the ability of the BIOMED-2 sets of primers to identify 2 clonal populations in the same formalin-fixed, paraffin-embedded tissue sample. Design.-Ten cases with 2 B-lymphoproliferative disorders at the same site were analyzed using 5 BIOMED-2 primer sets (IGH FR1, FR2, FR3, IGK VJ, and IGK VKde).

RESULTS:

All 10 cases (100%) showed at least 1 clone; 8 of 10 cases (80%) had 2 clones with at least 1 primer set, and the 2 clones were shown by 4 or 5 primer sets in none of the cases (0%), by 3 sets in 1 of 10 cases (10%), by 2 sets in 4 of 10 cases (40%), and by 1 set in 3 of 10 cases (30%). The most effective set was IGH FR2, detecting 4 of 10 biclonal cases (40%). The IGK VJ and IGK VKde each showed 2 clones in 3 of 10 cases (30% each). The least effective sets were IGH FR1 and FR3, with 2 of 10 cases (20%) each, with IGH FR1 being the least useful.

CONCLUSIONS:

The BIOMED-2 primers are effective in the detection of 2 clonal populations in the same sample.

• Authors: Brenda Ly, Claudiu V Cotta
• Journal: Archives of pathology & laboratory medicine (Arch Pathol Lab Med) Vol. 137 Issue 11 Pg. 1654-9 (Nov 2013) ISSN: 1543-2165 [Electronic] United States
• PMID: 24168505 (Publication Type: Journal Article)

Chemical References

• DNA Primers
• Immunoglobulin Heavy Chains
• Immunoglobulin kappa-Chains

Topics

• B-Lymphocytes (immunology, pathology)
• DNA Primers (genetics)
• Humans
• Immunoglobulin Heavy Chains (genetics)
• Immunoglobulin kappa-Chains (genetics)
• Immunophenotyping
• Lymphocytosis (diagnosis, genetics, immunology)
• Lymphoproliferative Disorders (diagnosis, genetics, immunology)
• Paraproteinemias (diagnosis, genetics, immunology)
• Polymerase Chain Reaction