Ganglioside GD2 as a therapeutic target for antibody-mediated therapy in patients with osteosarcoma.

Abstract

BACKGROUND:

Survival outcomes for patients with osteosarcoma have remained stagnant over the past 30 years. Targeting of ganglioside GD2, a glycosphingolipid on the cell surface of some tumors, with immunotherapy has resulted in improved outcomes for patients with neuroblastoma. In the current study, the expression pattern of GD2 was examined in osteosarcoma.

METHODS:

Immunohistochemistry was performed on osteosarcoma samples from patients at the time of initial biopsy, definitive surgery, and disease recurrence. The intensity and location of staining were scored. Cell-based enzyme-linked immunoadsorbent assay was performed on osteosarcoma cell lines to quantitate the level of GD2 expression.

RESULTS:

Forty-four osteosarcoma samples were evaluated by immunohistochemistry, including 8 samples from the initial biopsy, 28 samples from the definitive surgery, and 8 samples from the time of disease recurrence. GD2 was expressed on all 44 osteosarcoma samples. Osteosarcoma tissue obtained at the time of disease recurrence demonstrated a higher intensity of staining compared with samples obtained at initial biopsy and definitive surgery (P = .016). The majority of osteosarcoma cell lines expressed GD2 at higher levels than the neuroblastoma cell line BE(2)-C.

CONCLUSIONS:

Ganglioside GD2 is highly expressed on osteosarcomas. Clinical trials are needed to assess the efficacy of targeting GD2 in patients with osteosarcoma.

• Authors: Michael Roth, Marissa Linkowski, John Tarim, Sajida Piperdi, Rebecca Sowers, David Geller, Jonathan Gill, Richard Gorlick
• Journal: Cancer (Cancer) Vol. 120 Issue 4 Pg. 548-54 (Feb 15 2014) ISSN: 1097-0142 [Electronic] United States
• PMID: 24166473 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 American Cancer Society.

Chemical References

• Antibodies, Monoclonal
• Gangliosides
• ganglioside, GD2

Topics

• Antibodies, Monoclonal (therapeutic use)
• Biopsy
• Bone Neoplasms (drug therapy, genetics, pathology, surgery)
• Gangliosides (biosynthesis, genetics, immunology)
• Gene Expression Regulation, Neoplastic
• Humans
• Neoplasm Recurrence, Local (genetics, pathology, surgery)
• Neuroblastoma (genetics, pathology, surgery)
• Osteosarcoma (drug therapy, genetics, pathology, surgery)
• Primary Cell Culture
• Radioimmunotherapy