To improve the functional properties of peanut meal
protein for wide utilization, hydrolysis was conducted by
alcalase. Compared with saline and peanut meal
protein, intragastric administration of low molecular weight (<1 kD) peanut meal
peptide (PPH I) could significantly prolong swimming time, increase levels of
blood sugar, non-
esterified fatty acids (
NEFA) and
liver glycogen and decrease blood
lactate content in mice. Levels of
Pro, Leu, Val and His in low molecular weight peanut meal
peptides were higher significantly than those in other peanut meal
protein hydrolysates. Hydrophobic
amino acids, such as
Pro, Tyr and His, could perhaps capture
free radical and increase
antioxidant capacity of peanut
peptide and retard
fatigue induced by
free radical. After separation by HPLC, a primary
peptide P1,
Pro-Glu-Ile-Glu-Val, was sequenced. Its N-terminal was Val, and it was rich in
antioxidant amino acid, Pro and Ile. Levels of plasma
glucose,
NEFA and
liver glycogen in PPH I group were higher than those in mice intragastric administration with
peptide P1, and the swimming time is longer in PPH I group than in P1 group. So, the high content of P1 was one of the reason why PPH I had high endurance-enhancing capacity.