Cytotoxicity of triphenyltin(IV) methyl- and ethylisopropyldithiocarbamate compounds in chronic myelogenus leukemia cell line (K-562).

Abstract

UNLABELLED:

Studies on the discovery of new cancer treatment by using metal-based compounds such as tin (Sn) has now greatly being synthesized and evaluated to identify their effectiveness and suitability to be developed as a new anticancer drug.

APPROACH:

This study was carried out to evaluate the cytotoxicity of triphenyltin(lV) methylisopropyldithiocarbamate (compound 1) and triphenyltin(IV) ethylisopropyldithiocarbamate (compound (2) on chronic myelogenus leukemia cells. The determination of their cytotoxicity (IC50) at different time of exposure and concentration was carried out through the employment of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay.

RESULTS:

The IC50 values obtained for compound 1 and 2 following treatment at 24, 48 and 72 h were 0.660, 0.223, 0.370 microM and 0.677, 0.306, 0.360 microM, respectively. Cell morphological changes such as apoptotic and necrotic features were also been observed.

CONCLUSION:

The compounds tested were found to give cytotoxic effect against chronic myelogenus leukemia (K-562) cell at a micromolar dose. Thus, further study on their specific mechanism of actions in the human cells should be carried out to elucidate their potential as an anticancer agent.

• Authors: N Awang, N F Kamaludin, A Hamid, N W N Mokhtar, N F Rajab
• Journal: Pakistan journal of biological sciences : PJBS (Pak J Biol Sci) Vol. 15 Issue 17 Pg. 833-8 (Sep 01 2012) ISSN: 1028-8880 [Print] Pakistan
• PMID: 24163967 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Organotin Compounds
• Thiocarbamates
• triphenyltin

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Cell Shape (drug effects)
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Humans
• Inhibitory Concentration 50
• K562 Cells
• Leukemia, Myelogenous, Chronic, BCR-ABL Positive (pathology)
• Necrosis
• Organotin Compounds (pharmacology)
• Thiocarbamates (pharmacology)
• Time Factors