Synergistic stimulation of VIP/PHM-27 gene expression by cyclic AMP and phorbol esters in human neuroblastoma cells.

Abstract

The effects of dibutyryl cAMP (Bt2cAMP) and phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) and phorbol-12,13-didecanoate (PDD) on VIP/PHM-27 gene expression in human neuroblastoma cells in culture were investigated. Bt2cAMP and phorbol esters increased the VIP/PHM-27 mRNA level by about 9- and 4-fold, respectively. In the presence of both Bt2cAMP and phorbol esters, the VIP/PHM-27 mRNA level increased by about 36-fold. The intracellular cAMP level was essentially unaffected by phorbol esters. The VIP/PHM-27 gene dosage was unchanged by Bt2cAMP and phorbol esters. The results suggest that cAMP and phorbol esters synergistically induce the VIP/PHM-27 gene expression through independent pathways.

• Authors: K Ohsawa, Y Hayakawa, M Nishizawa, T Yamagami, H Yamamoto, N Yanaihara, H Okamoto
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 132 Issue 3 Pg. 885-91 (Nov 15 1985) ISSN: 0006-291X [Print] United States
• PMID: 2416313 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phorbol Esters
• RNA, Messenger
• Vasoactive Intestinal Peptide
• Bucladesine
• Cyclic AMP
• 1-Methyl-3-isobutylxanthine

Topics

• 1-Methyl-3-isobutylxanthine (pharmacology)
• Bucladesine (pharmacology)
• Cells, Cultured
• Cyclic AMP (analysis, pharmacology)
• Drug Synergism
• Gene Expression Regulation (drug effects)
• Humans
• Neuroblastoma (genetics)
• Phorbol Esters (pharmacology)
• RNA, Messenger (biosynthesis)
• Vasoactive Intestinal Peptide (genetics)