Abstract | BACKGROUND: Anti-angiogenic treatment of glioblastoma characteristically results in therapy resistance and tumor progression via diffuse infiltration. Monitoring tumor progression in these patients is thwarted because therapy results in tumor invisibility in contrast-enhanced (CE) MRI. To address this problem, we examined whether tumor progression could be monitored by metabolic mapping using (1)H MR spectroscopic imaging (MRSI). METHODS: RESULTS: In vivo imaging of choline/ n-acetyl aspartate ratios via multivoxel MRS is better able to evaluate response to therapy than CE-MRI. Lactate imaging revealed that diffuse infiltrative areas in glioblastoma xenografts did not present with excessive glycolysis. In contrast, glycolysis was observed in hypoxic areas in angiogenesis-dependent compact regions of glioma only, especially after anti-angiogenic treatment. CONCLUSION: Our data present MRSI as a powerful and feasible approach that is superior to CE-MRI and may provide handles for optimizing treatment of glioma. Furthermore, we show that glycolysis is more prominent in hypoxic areas than in areas of diffuse infiltrative growth. The Warburg hypothesis of persisting glycolysis in tumors under normoxic conditions may thus not be valid for diffuse glioma.
|
Authors | Bob Hamans, Anna Catharina Navis, Alan Wright, Pieter Wesseling, Arend Heerschap, William Leenders |
Journal | Neuro-oncology
(Neuro Oncol)
Vol. 15
Issue 12
Pg. 1615-24
(Dec 2013)
ISSN: 1523-5866 [Electronic] England |
PMID | 24158109
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Angiogenesis Inhibitors
- Contrast Media
- HIF1A protein, human
- Hypoxia-Inducible Factor 1, alpha Subunit
|
Topics |
- Angiogenesis Inhibitors
(therapeutic use)
- Animals
- Brain Neoplasms
(blood supply, diagnosis, drug therapy)
- Contrast Media
- Female
- Glioma
(blood supply, diagnosis, drug therapy)
- Glycolysis
- Humans
- Hypoxia
- Hypoxia-Inducible Factor 1, alpha Subunit
(metabolism)
- Immunoenzyme Techniques
- Magnetic Resonance Imaging
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Neovascularization, Pathologic
(diagnosis)
- Tumor Cells, Cultured
- Xenograft Model Antitumor Assays
|