Abstract | OBJECTIVES: METHODS: RESULTS: IPA in immunocompromised rats caused a dramatic increase in chitinase activity. This increase in chitinase activity was still noted when rats were treated with caspofungin. In vitro, it was demonstrated that the action of both chitinases were needed to lyse the fungal cell wall upon caspofungin exposure. CONCLUSION:
Caspofungin seemed to alter the cell wall in such a way that the two chitinases, when combined, could lyse the fungal cell wall and assisted in clearing the fungal pathogen. We also found that both chitinases combined had a direct effect on the fungus in vitro.
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Authors | Patricia E B Verwer, Marian T ten Kate, Franco H Falcone, Shaun Morroll, Henri A Verbrugh, Irma A J M Bakker-Woudenberg, Wendy W J van de Sande |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 10
Pg. e75848
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 24155872
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Echinocandins
- Lipopeptides
- Recombinant Proteins
- Hexosaminidases
- chitotriosidase
- Chitinases
- Caspofungin
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Topics |
- Animals
- Aspergillus fumigatus
(drug effects, physiology)
- Caspofungin
- Chitinases
(metabolism)
- Echinocandins
(pharmacology, therapeutic use)
- Hexosaminidases
(metabolism)
- Hyphae
(cytology, drug effects, metabolism)
- Immunocompromised Host
(drug effects)
- Invasive Pulmonary Aspergillosis
(drug therapy, microbiology)
- Lipopeptides
- Protein Binding
(drug effects)
- Rats
- Recombinant Proteins
(metabolism)
- Staining and Labeling
- Treatment Outcome
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