Screening study on hemolysis suppression effect of an alternative plasticizer for the development of a novel blood container made of polyvinyl chloride.

Abstract

The aim of this study is to identify a plasticizer that is effective in the suppression of the autohemolysis of the stored blood and can be used to replace di(2-ethylhexyl) phthalate (DEHP) in blood containers. The results of hemolysis test using mannitol-adenine-phosphate/red cell concentrates (MAP/RCC) spiked with plasticizers included phthalate, phthalate-like, trimeliate, citrate, and adipate derivatives revealed that di-isononyl-cyclohexane-1,2-dicarboxylate (Hexamoll(®) DINCH), di(2-ethylhexyl)-1,2,3,6-tetrahydro-phthalate (DOTP), and diisodecyl phthalate (DIDP) exhibited a hemolysis suppression effect almost equal to that of DEHP, but not other plasticizers. This finding suggested that the presence of 2 carboxy-ester groups at the ortho position on a 6-membered ring of carbon atoms may be required to exhibit such an effect. The hemolytic ratios of MAP/RCC-soaked polyvinyl chloride (PVC) sheets containing DEHP or different amounts of DINCH or DOTP were reduced to 10.9%, 9.2-12.4%, and 5.2-7.8%, respectively (MAP/RCC alone, 28.2%) after 10 weeks of incubation. The amount of plasticizer eluted from the PVC sheet was 53.1, 26.1-36.5, and 78.4-150 µg/mL for DEHP, DINCH, and DOTP, respectively. PVC sheets spiked with DIDP did not suppress the hemolysis induced by MAP/RCC because of low leachability (4.8-6.0 µg/mL). These results suggested that a specific structure of the plasticizer and the concentrations of least more than ∼10 µg/mL were required to suppress hemolysis due to MAP/RCC.

• Authors: Yuji Haishima, Tsuyoshi Kawakami, Chie Hasegawa, Akito Tanoue, Toshiyasu Yuba, Kazuo Isama, Atsuko Matsuoka, Shingo Niimi
• Journal: Journal of biomedical materials research. Part B, Applied biomaterials (J Biomed Mater Res B Appl Biomater) Vol. 102 Issue 4 Pg. 721-8 (May 2014) ISSN: 1552-4981 [Electronic] United States
• PMID: 24155207 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright © 2013 Wiley Periodicals, Inc.

Chemical References

• Benzoates
• Citrates
• Cyclohexanecarboxylic Acids
• Dicarboxylic Acids
• Inosine Nucleotides
• Oxazoles
• Plasticizers
• Pyrimidinones
• deoxyinosine diphosphate
• diisononyl 1,2-cyclohexanedicarboxylic acid
• 2,3-dihydro-5H-oxazolo(3,2-a)thieno(3,2-d)pyrimidin-5-one
• Mannitol
• citrate phosphate dextrose
• Polyvinyl Chloride
• Heparin
• trioctyl trimellitate
• Diethylhexyl Phthalate
• Glucose
• Adenine

Topics

• Adenine
• Benzoates (pharmacology)
• Blood Preservation (instrumentation)
• Citrates
• Cyclohexanecarboxylic Acids (pharmacology)
• Depression, Chemical
• Dicarboxylic Acids (pharmacology)
• Diethylhexyl Phthalate (pharmacology, toxicity)
• Gas Chromatography-Mass Spectrometry
• Glucose
• Hemolysis (drug effects)
• Heparin
• Humans
• Inosine Nucleotides (pharmacology)
• Mannitol
• Oxazoles (pharmacology)
• Plasticizers (chemistry, pharmacology)
• Polyvinyl Chloride
• Pyrimidinones (pharmacology)
• Structure-Activity Relationship