Daidzein and its
glycoside form
daidzin, are known to have potential health benefits and are metabolized to O‑desmethylangolensin (O‑DMA) and
equol following consumption. In the current study, the
antioxidant activity and cytotoxicity of O‑DMA,
equol,
daidzein and
daidzin was investigated and their effects on HepG2 human hepatocelluar
carcinoma cells were compared. For cytotoxicity assays,
lactose dehydrogenase (LDH) release and 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide‑based cell viability, cells were exposed to various concentrations of each compound (5‑200 µM) for 24, 48 or 72 h. O‑DMA and
equol did not affect LDH release, but higher concentrations (<75 µM) showed inhibition of cell growth. By contrast,
daidzein and
daidzin (200 µM) increased LDH release and cell growth. All compounds stimulated
catalase and total
superoxide dismutase (SOD) (CuZn‑ and Mn‑SOD) activity, and
mRNA and
protein expression. This phenomenon was most pronounced for O‑DMA and
equol, as their effects were similar. These data suggested that O‑DMA and
equol possess greater
antioxidant properties compared with
daidzein and may, thus, be beneficial for human health.