[Effects of Xixin decoction on enzymes related to O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer's disease].

Abstract

OBJECTIVE:

To study the effects of Xixin decoction (XXD) on O-GlcNAc transferase (OGT) and O-GlcNAc glycosidase in O-GlcNAc glycosylation of tau proteins in the brain of rats with sporadic Alzheimer's disease (SAD) and explore the possible mechanism.

METHODS:

Male SD rats were randomly divided into sham-operated group, model group, donepezil group, and low-, moderate-, and high-dose XXD groups. After treatment and behavioral test, the rats were sacrificed for detecting the expressions of OGT and O-GlcNAc glycosidase in the brain using immunohistochemistry and Western blotting.

RESULTS:

XXD significantly enhanced the expressions of OGT in the hippocampus of SAD rats and lowered the expression of O-GlcNAc glycosidase (P<0.05 or 0.01). OGT and O-GlcNAc glycosidase expressions showed no significant differences between the model group and donepezil group (P>0.05).

CONCLUSION:

XXD can regulate the expression of OGT and O-GlcNAc glycosidase to enhance O-GlcNAc glycosylation of tau proteins in the hippocampus of SAD rats.

• Authors: Yongchang Diwu, Jinzhou Tian, Jing Shi
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 33 Issue 10 Pg. 1442-7 (Oct 2013) ISSN: 1673-4254 [Print] China
• PMID: 24144743 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• tau Proteins
• Streptozocin
• N-Acetylglucosaminyltransferases
• O-GlcNAc transferase
• Acetylglucosaminidase

Topics

• Acetylglucosaminidase (metabolism)
• Alzheimer Disease (chemically induced, enzymology, metabolism)
• Animals
• Dose-Response Relationship, Drug
• Drugs, Chinese Herbal (administration & dosage, pharmacology)
• Glycosylation
• Hippocampus (enzymology, metabolism)
• Male
• N-Acetylglucosaminyltransferases (metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Streptozocin
• tau Proteins (metabolism)