Nitric oxide metabolites during anoxia and reoxygenation in the anoxia-tolerant vertebrate Trachemys scripta.

Abstract

Moderate elevations of nitrite and nitric oxide (NO) protect mammalian tissues against ischemia (anoxia)-reperfusion damage by inhibiting mitochondrial electron transport complexes and reducing the formation of reactive oxygen species (ROS) upon reoxygenation. Crucian carp appear to exploit this mechanism by upregulating nitrite and other nitrite/NO metabolites (S-nitroso and iron-nitrosyl compounds) in several tissues when exposed to anoxia. We investigated whether this is a common strategy amongst anoxia-tolerant vertebrates by evaluating NO metabolites in red-eared slider turtles during long-term (9 days) anoxia and subsequent reoxygenation at low temperature, a situation naturally encountered by turtles in ice-covered ponds. We also measured glutathione in selected tissues and assessed the impact of anoxia on electrolyte status. Anoxia induced major increases in [nitrite] in the heart, pectoral muscle and red blood cells, while [nitrite] was maintained unaltered in brain and liver. Concomitantly, the concentrations of S-nitroso and iron-nitrosyl compounds increased, showing that nitrite was used to produce NO and to S-nitrosate cellular molecules during anoxia. The changes were gradually reversed during reoxygenation (1 h and 24 h), testifying that the processes were reversible. The increased NO bioavailability occurred in the absence of NO synthase activity (due to global anoxia) and may involve mobilization of internal/external nitrite reservoirs. Our data support the theory that anoxic upregulation of nitrite and other NO metabolites could be a general cytoprotective strategy amongst anoxia-tolerant vertebrates. The possible mechanisms of nitrite-derived NO and S-nitrosation in protecting cells from destructive Ca(2+) influx during anoxia and in limiting ROS formation during reoxygenation are discussed.

• Authors: Frank B Jensen, Marie N Hansen, Gabriella Montesanti, Tobias Wang
• Journal: The Journal of experimental biology (J Exp Biol) Vol. 217 Issue Pt 3 Pg. 423-31 (Feb 01 2014) ISSN: 1477-9145 [Electronic] England
• PMID: 24143029 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Nitrites
• Nitric Oxide
• Oxygen

Topics

• Animals
• Cytoprotection
• Erythrocytes (metabolism)
• Hypoxia (blood, metabolism)
• Nitric Oxide (blood, metabolism)
• Nitrites (metabolism)
• Oxygen (blood, metabolism)
• Turtles (blood, metabolism)