Synthesis and characterization of lipid immuno-nanocapsules for directed drug delivery: selective antitumor activity against HER2 positive breast-cancer cells.

Abstract

Lipid nanocapsules (LNC) are usually developed as nanocarriers for lipophilic drug delivery. The surface characteristics of these colloidal particles are determinant for a controlled and directed delivery to target tissues with specific markers. We report the development of immuno-nanocapsules, in which some antibody molecules with different immuno-specificity are conjugated to the nanocapsule surface, offering the standardization of a simple method to obtain vectorized nanosystems with specific recognition properties. Nanocapsules were prepared by a solvent-displacement technique, producing an oily core coated by a functional shell of different biocompatible molecules and surface carboxylic groups. Three different antibodies (one a specific HER2 oncoprotein antibody) were conjugated with these nanoparticles by the carbodiimide method, which allows the covalent immobilization of protein molecules through carboxylic surface groups. The immuno-nanocapsules were completely characterized physico-chemically via electrokinetic and colloidal stability experiments, confirming the correct immobilization of these antibody molecules on the colloidal nanoparticles. Also, additional immunological analyses verified that these IgG-LNC complexes showed the expected specific immuno-response. Finally, different healthy and tumoral breast-cell lines were cultured in vitro with Nile-Red-loaded and docetaxel-loaded HER2 immuno-nanocapsules. The results indicate that our immuno-nanocapsules can increase their uptake in HER2 overexpressing tumoral cell lines.

• Authors: Paola Sánchez-Moreno, Juan Luis Ortega-Vinuesa, Houría Boulaiz, Juan Antonio Marchal, José Manuel Peula-García
• Journal: Biomacromolecules (Biomacromolecules) Vol. 14 Issue 12 Pg. 4248-59 (Dec 09 2013) ISSN: 1526-4602 [Electronic] United States
• PMID: 24134122 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal, Humanized
• Antineoplastic Agents
• Colloids
• Nanocapsules
• Olive Oil
• Plant Oils
• Taxoids
• Deoxycholic Acid
• Poloxamer
• Docetaxel
• ERBB2 protein, human
• Receptor, ErbB-2
• Trastuzumab

Topics

• Antibodies, Monoclonal, Humanized (chemistry, metabolism, pharmacology)
• Antineoplastic Agents (chemistry, metabolism, pharmacology)
• Breast Neoplasms
• Colloids
• Deoxycholic Acid (chemistry)
• Docetaxel
• Drug Screening Assays, Antitumor
• Female
• Humans
• Inhibitory Concentration 50
• MCF-7 Cells
• Nanocapsules (chemistry)
• Olive Oil
• Particle Size
• Plant Oils (chemistry)
• Poloxamer (chemistry)
• Receptor, ErbB-2 (metabolism)
• Taxoids (chemistry, metabolism, pharmacology)
• Trastuzumab