A visible light photoelectrochemical sensor for tumor marker detection using tin dioxide quantum dot-graphene as labels.
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| Abstract |
In this paper, a simple and sensitive sandwich-type photoelectrochemical (PEC) immunosensor for measurement of biomarkers on a gold nanoparticle-modified indium tin oxide (ITO) electrode through electrodeposition for point-of-care testing was developed by using a tin dioxide quantum dot-graphene nanocomposite (G-SnO2) as an excellent label with amplification techniques. The capture antibody (Ab1) was firstly immobilized on the gold nanoparticle-modified ITO electrode due to the covalent conjugation, then the antigen and the AuNP/PDDA-G-SnO2 nanocomposite nanoparticle labeled signal antibody (Ab2) were conjugated successively to form a sandwich-type immunocomplex through a specific interaction. Under irradiation with a common ultraviolet lamp (∼365 nm, price $50), the SnO2 NPs were excited and underwent charge-separation to yield electrons (e(-)) and holes (h(+)). As the holes were scavenged by ascorbic acid (AA), the electrons were transferred to the ITO electrode through RGO to generate a photocurrent. The photocurrents were proportional to the CEA concentrations, and the linear range of the developed immunosensor was from 0.005 to 10 ng mL(-1) with a detection limit of 0.036 pg mL(-1). The proposed sensor shows high sensitivity, stability, reproducibility, and can become a promising platform for other biomolecular detection.
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| Authors | Yanhu Wang, Meng Li, Yuanna Zhu, Shenguang Ge, Jinghua Yu, Mei Yan, Xianrang Song |
| Journal | The Analyst (Analyst) Vol. 138 Issue 23 Pg. 7112-8 (Dec 07 2013) ISSN: 1364-5528 [Electronic] England |
| PMID | 24133676 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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