Abstract |
This study investigated the neuronal protective effect of monosialotetrahexosylganglioside (GM1) on the hypoxia- ischemia white matter damage (WMD) in neonatal rats. Brain hypoxia-ischemia was induced by bilateral carotid artery occlusion in 4-day-old neonatal rats. Bilateral carotid artery occlusion (BCAO) was performed in rats in WMD and GM1 groups, while in sham group; the rat bilateral carotid arteries were merely exposed without occlusion. Immunohistochemical staining was used to determine the expression of myelin basic protein (MBP), glial fibrillary acidic protein (GFAP), and beta-amyloid precursor protein (beta-APP). In addition, suspension test, slope test, and open-field test were carried out on day 26 after BCAO to determine the neurobehavioral function. The percentage of MBP-positive cells was decreased while beta-APP-and GFAPpositive cells were increased in WMD group. After treated with GM1, the percentage of MBP-positive cells increased significantly than WMD rats at post-operation 72 h and day 7. GFAP-positive cells and beta-APP-positive cells decreased significantly in WMD group at post-operation 72 h, day 7 and 26. The suspension test, slope test, and open-field test showed that neurobehavioral function was improved in ganglioside GM1 group compared with WMD group. Taken together, our findings suggested that ganglioside GM1 treatment reduces hypoxia- ischemia induced impairment of the neurobehavioral function in WMD in neonatal rats.
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Authors | Xiao Rong, Wei Zhou, Chen Xiao-Wen, Li Tao, Juan Tang |
Journal | Acta neurobiologiae experimentalis
(Acta Neurobiol Exp (Wars))
Vol. 73
Issue 3
Pg. 379-86
( 2013)
ISSN: 1689-0035 [Electronic] Poland |
PMID | 24129486
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amyloid beta-Protein Precursor
- Glial Fibrillary Acidic Protein
- Myelin Basic Protein
- G(M1) Ganglioside
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Topics |
- Amyloid beta-Protein Precursor
(metabolism)
- Animals
- Animals, Newborn
- Carotid Artery Diseases
(complications)
- Disease Models, Animal
- Female
- G(M1) Ganglioside
(therapeutic use)
- Gene Expression Regulation
(drug effects)
- Glial Fibrillary Acidic Protein
(metabolism)
- Leukoencephalopathies
(drug therapy, etiology)
- Male
- Myelin Basic Protein
(metabolism)
- Neurons
(drug effects, metabolism, pathology)
- Rats
- Rats, Sprague-Dawley
- Time Factors
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