Chlorella 11-peptide inhibits the production of macrophage-induced adhesion molecules and reduces endothelin-1 expression and endothelial permeability.

Abstract

The inflammation process in large vessels involves the up-regulation of vascular adhesion molecules such as endothelial cell selectin (E-selectin), intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) which are also known as the markers of atherosclerosis. We have reported that Chlorella 11-peptide exhibited effective anti-inflammatory effects. This peptide with an amino sequence Val-Glu-Cys-Tyr-Gly-Pro-Asn-Arg-Pro-Gln-Phe was further examined for its potential in preventing atherosclerosis in this study. In particular, the roles of Chlorella 11-peptide in lowering the production of vascular adhesion molecules, monocyte chemoattractant protein (MCP-1) and expression of endothelin-1 (ET-1) from endothelia (SVEC4-10 cells) were studied. The production of E-selectin, ICAM-1, VCAM-1 and MCP-1 in SVEC4-10 cells was measured with ELISA. The mRNA expression of ET-1 was analyzed by RT-PCR and agarose gel. Results showed that Chlorella 11-peptide significantly suppressed the levels of E-selectin, ICAM, VCAM, MCP-1 as well as ET-1 gene expression. The inhibition of ICAM-1 and VCAM-1 production by Chlorella 11-peptide was reversed in the presence of protein kinase A inhibitor (H89) which suggests that the cAMP pathway was involved in the inhibitory cause of the peptide. In addition, this peptide was shown to reduce the extent of increased intercellular permeability induced by combination of 50% of lipopolysaccharide (LPS)-activated RAW 264.7 cells medium and 50% normal SEVC cell culture medium (referred to as 50% RAW-conditioned medium). These data demonstrate that Chlorella 11-peptide is a promising biomolecule in preventing chronic inflammatory-related vascular diseases.

• Authors: Mei Fen Shih, Lih Chi Chen, Jong Yuh Cherng
• Journal: Marine drugs (Mar Drugs) Vol. 11 Issue 10 Pg. 3861-74 (Oct 14 2013) ISSN: 1660-3397 [Electronic] Switzerland
• PMID: 24129228 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ccl2 protein, mouse
• Cell Adhesion Molecules
• Chemokine CCL2
• E-Selectin
• Endothelin-1
• Peptides
• Val-Glu-Cys-Tyr-Gly-Pro-Asn-Arg-Pro-Gln-Phe
• Vascular Cell Adhesion Molecule-1
• Intercellular Adhesion Molecule-1

Topics

• Animals
• Atherosclerosis (genetics, metabolism, prevention & control)
• Cell Adhesion Molecules (genetics, metabolism)
• Cell Line
• Chemokine CCL2 (genetics, metabolism)
• Chlorella (chemistry)
• E-Selectin (genetics, metabolism)
• Endothelin-1 (genetics, metabolism)
• Endothelium (drug effects, metabolism)
• Intercellular Adhesion Molecule-1 (genetics, metabolism)
• Macrophages (drug effects, metabolism)
• Mice
• Peptides (pharmacology)
• Permeability (drug effects)
• Vascular Cell Adhesion Molecule-1 (genetics, metabolism)