The microchromatographic measurement of fetal hemoglobin levels in hemoglobin C-associated conditions.

Abstract

We report the microchromatographic estimation of fetal hemoglobin (Hb F) in 3 conditions associated with hemoglobin C: hemoglobin C-hereditary persistence of fetal hemoglobin (Hb C-HPFH), homozygous hemoglobin C disease, and hemoglobin SC disease. In the case of Hb C-HPFH (Case 1) the Hb F level by alkali denaturation was 32.5% while the Hb F level by microchromatography was 35.3%. In both cases of homozygous Hb C disease and Hb SC disease the Hb F level by alkali denaturation was less than 1% and the microchromatographic Hb F level was 0.7%. We conclude that microchromatographic methods for determination of Hb F levels can be employed in the Hb C-HPFH, homozygous Hb C, and Hb SC conditions. To our knowledge, the microchromatographic determination of Hb F levels has not been applied previously to the Hb C-HPFH condition or Hb C disease.

• Authors: J S Krauss, M H Jonah, C R Baisden, D Y Parnell, A E Neely, P Mohorn
• Journal: Clinical biochemistry (Clin Biochem) Vol. 18 Issue 4 Pg. 252-3 (Aug 1985) ISSN: 0009-9120 [Print] United States
• PMID: 2412727 (Publication Type: Case Reports, Journal Article)

Chemical References

• Hemoglobin C
• Fetal Hemoglobin

Topics

• Adolescent
• Adult
• Chromatography, Ion Exchange (methods)
• Electrophoresis, Cellulose Acetate (methods)
• Fetal Hemoglobin (analysis)
• Hemoglobin C (analysis)
• Humans
• Immunodiffusion
• Male
• Spectrophotometry