Abstract |
We report the microchromatographic estimation of fetal hemoglobin (Hb F) in 3 conditions associated with hemoglobin C: hemoglobin C-hereditary persistence of fetal hemoglobin (Hb C-HPFH), homozygous hemoglobin C disease, and hemoglobin SC disease. In the case of Hb C-HPFH (Case 1) the Hb F level by alkali denaturation was 32.5% while the Hb F level by microchromatography was 35.3%. In both cases of homozygous Hb C disease and Hb SC disease the Hb F level by alkali denaturation was less than 1% and the microchromatographic Hb F level was 0.7%. We conclude that microchromatographic methods for determination of Hb F levels can be employed in the Hb C-HPFH, homozygous Hb C, and Hb SC conditions. To our knowledge, the microchromatographic determination of Hb F levels has not been applied previously to the Hb C-HPFH condition or Hb C disease.
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Authors | J S Krauss, M H Jonah, C R Baisden, D Y Parnell, A E Neely, P Mohorn |
Journal | Clinical biochemistry
(Clin Biochem)
Vol. 18
Issue 4
Pg. 252-3
(Aug 1985)
ISSN: 0009-9120 [Print] United States |
PMID | 2412727
(Publication Type: Case Reports, Journal Article)
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Chemical References |
- Hemoglobin C
- Fetal Hemoglobin
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Topics |
- Adolescent
- Adult
- Chromatography, Ion Exchange
(methods)
- Electrophoresis, Cellulose Acetate
(methods)
- Fetal Hemoglobin
(analysis)
- Hemoglobin C
(analysis)
- Humans
- Immunodiffusion
- Male
- Spectrophotometry
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