Effects of modified Sanhuang decoction () enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats.

Abstract

OBJECTIVE:

To investigate the effects of Modified Sanhuang Decoction (, MSD) enema on the serum tumor necrosis factor alpha (TNF-α) and colonic mucosa interleukin-1β (IL-1β), interleukin-6 (IL-6) levels in experimental ulcerative colitis (UC) rats.

METHODS:

Forty-five male Wistar rats were randomly divided into 4 groups: normal group (n=12), model group (n=11), salazosulfapyridine (SASP) group (n=11) and MSD group (n=11). The UC model was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS)/ethanol solution. Rats in the normal group and model group were clystered with 0.9% normal saline, while in the SASP group and MSD group were clystered with SASP and MSD enema, respectively. After drug administration (10 mL/kg body weight, for 7 days), colonic gross changes and colonic mucosa histology were observed, serum TNF-α and colonic mucosa IL-1β, IL-6 levels were tested by enzyme linked immunosorbent assay and radioimmunoassay, respectively.

RESULTS:

As compared with the normal group, the experimental UC rats, the colonic mucosal damage index scores (CMDIs), histopathological scores (HS) and the serum TNF-α and colonic mucosa IL-1β, IL-6 levels significantly increased (P<0.05 or P<0.01). In the MSD and SASP groups, the ulcer area significantly reduced, and edema disappeared. The CMDIs, HS, the serum TNF-α and colonic mucosa IL-1β, IL-6 levels in the MSD and SASP groups significantly decreased (P<0.05 or P<0.01) compared with the model group. The CMDIs in the MSD group were lower than that in the SASP group (P<0.05), but there were no significant differences in HS, serum TNF-α or colonic mucosa IL-1β, IL-6 levels between the MSD and SASP groups.

CONCLUSION:

MSD enema can improve colonic mucosa impairment and decrease serum TNF-α and colonic mucosa IL-1β, IL-6 levels in experimental UC.

• Authors: Shuai Wang, Tao Zhou, Jun-Peng Zhai, Li-Hua Wang, Jing Chen
• Journal: Chinese journal of integrative medicine (Chin J Integr Med) Vol. 20 Issue 11 Pg. 865-9 (Nov 2014) ISSN: 1672-0415 [Print] China
• PMID: 24126972 (Publication Type: Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Interleukin-1beta
• Interleukin-6
• sanhuang

Topics

• Animals
• Colitis, Ulcerative (metabolism, therapy)
• Colon (metabolism)
• Drugs, Chinese Herbal (administration & dosage)
• Enema
• Interleukin-1beta (blood, metabolism)
• Interleukin-6 (blood, metabolism)
• Intestinal Mucosa (metabolism)
• Male
• Rats
• Rats, Wistar