Repression of cell proliferation and androgen receptor activity in prostate cancer cells by 2'-hydroxyflavanone.

Abstract

BACKGROUND:

Prevention of the development of castration-resistant from hormone-naïve prostate cancer is an important issue in maintaining the quality of life of the patients. We explored the effect of 2'-hydroxyflavanone on proliferation and androgen responsiveness using prostate cancer cell lines.

MATERIALS AND METHODS:

To investigate the effect of 2'-hydroxyflavanone on proliferation, prostate cancer cells were treated with 2'-hydroxyflavanone. Androgen-responsiveness in LNCaP cells was confirmed by luciferase assay after transfection of luciferase reporter driven by prostate specific antigen promoter. To detect androgen receptor (AR) expression, reverse transcriptase polymerase chain reaction and western blot analysis were conducted.

RESULTS:

2'-Hydroxyflavanone inhibited the proliferation of PC-3 and DU145 cells by induction of apoptosis. 2'-Hydroxyflavanone inhibited the proliferation of LNCaP cells stimulated by androgens and attenuated androgen-responsiveness through down-regulation of AR protein.

CONCLUSION:

2'-Hydroxyflavanone not only inhibited proliferation of prostate cancer cells, but also repressed androgen-responsiveness, suggesting that it might be a useful agent in preventing recurrence of prostate cancer.

• Authors: Mitsuo Ofude, Atsushi Mizokami, Misako Kumaki, Kouji Izumi, Hiroyuki Konaka, Yoshifumi Kadono, Yasuhide Kitagawa, Minkyoung Shin, Jian Zhang, Evan T Keller, Mikio Namiki
• Journal: Anticancer research (Anticancer Res) Vol. 33 Issue 10 Pg. 4453-61 (Oct 2013) ISSN: 1791-7530 [Electronic] Greece
• PMID: 24123015 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 2'-hydroxyflavanone
• Androgens
• Antineoplastic Agents, Hormonal
• Flavanones
• Receptors, Androgen
• Androstenedione
• Estradiol Dehydrogenases
• 3-Oxo-5-alpha-Steroid 4-Dehydrogenase
• KLK3 protein, human
• Kallikreins
• Prostate-Specific Antigen

Topics

• 3-Oxo-5-alpha-Steroid 4-Dehydrogenase (metabolism)
• Active Transport, Cell Nucleus (drug effects)
• Androgens (biosynthesis)
• Androstenedione (pharmacology)
• Antineoplastic Agents, Hormonal (pharmacology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Drug Screening Assays, Antitumor
• Estradiol Dehydrogenases (metabolism)
• Flavanones (pharmacology)
• Gene Expression
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Kallikreins (genetics, metabolism)
• Male
• Promoter Regions, Genetic
• Prostate-Specific Antigen (genetics, metabolism)
• Prostatic Neoplasms, Castration-Resistant
• Receptors, Androgen (genetics, metabolism)