Chemical and immunochemical characterisation of substance P-like immunoreactivity and physalaemin-like immunoreactivity in a carcinoid tumour.

Abstract

Extracts of a carcinoid tumour, resected from the mid-portion of the ileum of a patient with no symptoms of endocrine disorder, were associated with a high concentration of substance P-like immunoreactivity. Using reverse-phase high performance liquid chromatography and antisera specifically directed against the C-terminal and N-terminal regions of substance P and against the N-terminal region of physalaemin, the following components were isolated and identified: substance P and its oxidised form, [pGlu5]substance P-(5-11) peptide and its oxidised form, and the oxidised form of physalaemin. The identity of tumour substance P with the undecapeptide was confirmed by amino acid analysis and high performance ion-exchange chromatography. In vitro incubation of tumour tissue from a lymph node metastasis from the same patient with [3H]leucine resulted in incorporation of radioactivity into newly synthesised substance P.

• Authors: J M Conlon, G Schäfer, W E Schmidt, L H Lazarus, H D Becker, W Creutzfeldt
• Journal: Regulatory peptides (Regul Pept) Vol. 11 Issue 2 Pg. 117-32 (Jun 1985) ISSN: 0167-0115 [Print] Netherlands
• PMID: 2412263 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Kinins
• Physalaemin
• Substance P

Topics

• Carcinoid Tumor (analysis, blood, pathology)
• Chromatography, High Pressure Liquid
• Cross Reactions
• Humans
• Ileal Neoplasms (analysis, blood, pathology)
• Kinins (analysis)
• Male
• Middle Aged
• Physalaemin (analysis)
• Substance P (analysis, biosynthesis, blood)