Inhibition of lung inflammatory responses by bornyl acetate is correlated with regulation of myeloperoxidase activity.

Abstract

BACKGROUND:

Bornyl acetate is a bicyclic monoterpene present in numerous conifer oils. In this study, we aimed at clarifying the potential anti-inflammatory function and mechanism of bornyl acetate by using lipopolysaccharide (LPS)-induced acute lung injury murine model and RAW 264.7 cells.

MATERIALS AND METHODS:

RAW 264.7 cells were pretreated with bornyl acetate 1 h before LPS stimulation and cell-free super supernatants were collected to measure cytokine concentrations. To induce acute lung injury, BALB/c mice were injected intranasally with LPS and treated with bornyl acetate 1 h before LPS stimulation. Seven hours after administration, the bronchoalveolar lavage fluid (BALF) was collected for measuring the cell count and cytokine production. We collected lungs for assaying wet-to-dry weight ratio, myeloperoxidase activity, and histologic changes. The extent of phosphorylation of mitogen-activated protein kinases and nuclear factor κB was detected by Western blot.

RESULTS:

Our results showed that bornyl acetate downregulated the levels of proinflammatory cytokines in vitro and in vivo; reduced the number of total cells, neutrophils, and macrophages in BALF; attenuated the histologic alterations in the lung; decreased the wet-to-dry weight ratio in BALF; and suppressed NF-kappa-B inhibitor alpha, extracellular regulated protein kinases, c-JunN-terminal kinase, p38 mitogen-activated protein kinase activation.

CONCLUSIONS:

These findings suggested that bornyl acetate may be developed as a preventive agent for lung inflammatory diseases.

• Authors: Na Chen, Guoquan Sun, Xue Yuan, Jinli Hou, Qianchao Wu, Lanan W Soromou, Haihua Feng
• Journal: The Journal of surgical research (J Surg Res) Vol. 186 Issue 1 Pg. 436-45 (Jan 2014) ISSN: 1095-8673 [Electronic] United States
• PMID: 24120240 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Camphanes
• Cytokines
• NF-kappa B
• bornyl acetate
• Peroxidase

Topics

• Acute Lung Injury (pathology)
• Animals
• Camphanes (pharmacology)
• Cells, Cultured
• Cytokines (biosynthesis)
• Lung (drug effects, pathology)
• MAP Kinase Signaling System (drug effects)
• Macrophages (drug effects, physiology)
• Male
• Mice
• Mice, Inbred BALB C
• NF-kappa B (metabolism)
• Peroxidase (metabolism)
• Pneumonia (prevention & control)