Panaxatriol saponin ameliorated liver injury by acetaminophen via restoring thioredoxin-1 and pro-caspase-12.

Abstract

BACKGROUND & AIMS:

Acetaminophen (APAP) is widely used as an antipyretic agent which is safe at therapeutic doses. However, overdose of APAP induces fatal and non-fatal hepatic necroses. The chemical reactive metabolites of APAP initiate toxicity and inflammatory response within the liver and lead to acute liver failure. However, the mechanism underlying APAP-induced liver injury is unknown. Thioredoxin-1 (TRX-1) is an important redox regulator, which plays roles in resisting oxidative stress, regulating inflammation and inhibiting apoptosis. Panaxatriol saponin (PTS) is one of the biologically active fractions of Panax notoginseng which is a traditional Chinese medicine. The aim of this study was to investigate the mechanism on PTS protecting liver from APAP hepatotoxicity.

METHODS:

Mice were divided into three groups, control group, APAP group and APAP combined with PTS group. Alanine aminotransferase (ALT) and tumour necrosis factor-alpha (TNF-α) were detected by ELISA. TRX-1 and pro-caspase-12 were examined by Western blotting.

RESULTS:

Our results showed PTS inhibited the levels of ALT and TNF-α by APAP. Pretreatment with PTS ameliorated liver injury induced by APAP. The decrease in TRX-1 expression was restored by PTS, as well as decreased pro-caspase-12 expression was inhibited by PTS. These data suggest that PTS has roles in suppressing the hepatotoxicity by APAP.

CONCLUSION:

Panaxatriol saponin ameliorated liver injury by APAP through restoring the expression TRX-1 and inhibiting pro-caspase-12 decrease.

• Authors: Shengdong Wang, Xiao Wang, Fucheng Luo, Xiaodan Tang, Kui Li, Xiaomei Hu, Jie Bai
• Journal: Liver international : official journal of the International Association for the Study of the Liver (Liver Int) Vol. 34 Issue 7 Pg. 1068-73 (Aug 2014) ISSN: 1478-3231 [Electronic] United States
• PMID: 24119161 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Ginsenosides
• Tumor Necrosis Factor-alpha
• Txn1 protein, mouse
• Acetaminophen
• Thioredoxins
• panaxatriol
• Alanine Transaminase
• Caspase 12

Topics

• Acetaminophen (adverse effects)
• Alanine Transaminase (blood)
• Analysis of Variance
• Animals
• Blotting, Western
• Caspase 12 (metabolism)
• Chemical and Drug Induced Liver Injury (drug therapy)
• Enzyme-Linked Immunosorbent Assay
• Ginsenosides (pharmacology, therapeutic use)
• Mice
• Thioredoxins (metabolism)
• Tumor Necrosis Factor-alpha (blood)