Abstract | BACKGROUND: METHODS: The expression of miR-26a was examined in 15 pairs of pancreatic duct adenocarcinoma (PDAC) and their adjacent benign pancreatic tissues (ABPT), by qRT-PCR. The results were confirmed by in situ hybridization using two panels of 106 PDACs and their ABPT microarray. The association of miR-26a expression with overall survival was determined. The proliferation and cell cycle distribution of Capan-2, SW-1990, and Panc-1 cells, transfected with miR-26a mimics or a miR-26a inhibitor, were assessed using the Cell Counting Kit-8 assay and flow cytometry, respectively. The cell tumorigenicity was evaluated via murine xenograft experiments. Cyclin D2, E2, EZH2, and PCNA levels were analyzed by Western blot and immunohistochemistry. RESULTS: miR-26a was expressed in the cytoplasm of pancreatic ductal epithelial cells, whereas its expression was significantly downregulated in PDAC tissues compared with that of ABPT. Patients with low miR-26a expression had a significantly shorter survival than those with high miR-26a expression. The in vitro and in vivo assays showed that overexpression of miR-26a resulted in cell cycle arrest, inhibited cell proliferation, and decreased tumor growth, which was associated with cyclin E2 downregulation. CONCLUSIONS:
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Authors | Jingjing Deng, Miaoxia He, Lizao Chen, Chao Chen, Jianming Zheng, Zailong Cai |
Journal | PloS one
(PLoS One)
Vol. 8
Issue 10
Pg. e76450
( 2013)
ISSN: 1932-6203 [Electronic] United States |
PMID | 24116110
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CCNE2 protein, human
- Cyclins
- MIRN26A microRNA, human
- MicroRNAs
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Topics |
- Adenocarcinoma
(genetics, metabolism, pathology)
- Animals
- Blotting, Western
- Carcinoma, Pancreatic Ductal
(genetics, metabolism, pathology)
- Cell Line, Tumor
- Cell Proliferation
- Cyclins
(genetics, metabolism)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- In Situ Hybridization
- Kaplan-Meier Estimate
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- MicroRNAs
(genetics)
- Middle Aged
- Pancreatic Neoplasms
(genetics, metabolism, pathology)
- RNA Interference
- Reverse Transcriptase Polymerase Chain Reaction
- Tissue Array Analysis
- Transplantation, Heterologous
- Tumor Burden
(genetics)
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