Sensitizing potential of enzymatically cross-linked peanut proteins in a mouse model of peanut allergy.

Abstract

SCOPE:

The cross-linking of proteins by enzymes to form high-molecular-weight protein, aggregates can be used to tailor the technological or physiological functionality of food products. Aggregation of dietary proteins by food processing may promote allergic sensitization, but the effects of enzymatic cross-linking of dietary proteins on the allergenic potential of food are not known. In this study, the bioavailability and the sensitizing or tolerizing potential of peanut proteins (PE) cross-linked with microbial tyrosinase from Trichoderma reesei and mushroom tyrosinase from Agaricus bisporus, were investigated.

METHODS AND RESULTS:

The impact of cross-linking of PE on the in vitro bioavailability of fluorescein isothiocyanate-labeled peanut proteins was tested in a Caco-2 cell monolayer and by competitive ELISA. The in vivo allergenicity or capacity to induce oral tolerance in mice were measured by serum levels of PE-specific antibodies and T cell cytokine production after exposure to PE and cross-linked PE.

CONCLUSION:

Enzymatic processing of peanut proteins by the two tyrosinases increased the bioavailability of major peanut allergen Ara h 2, but did not significantly change the allergenic or tolerizing properties of peanut. Enzymatic treatment of peanut proteins yielded cross-linked proteins with preserved molecular and immunological features of peanut allergens.

• Authors: Jelena Radosavljevic, Emilia Nordlund, Luka Mihajlovic, Maja Krstic, Torsten Bohn, Johanna Buchert, Tanja Cirkovic Velickovic, Joost Smit
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 58 Issue 3 Pg. 635-46 (Mar 2014) ISSN: 1613-4133 [Electronic] Germany
• PMID: 24115517 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• 2S Albumins, Plant
• Antigens, Plant
• Ara h 2 allergen, Arachis hypogaea
• Ara h 6 allergen, Arachis hypogaea
• Cross-Linking Reagents
• Epitopes
• Glycoproteins
• Plant Proteins
• Immunoglobulin E
• Monophenol Monooxygenase

Topics

• 2S Albumins, Plant (metabolism)
• Animals
• Antigens, Plant (metabolism)
• Arachis (immunology)
• Biological Availability
• Caco-2 Cells
• Cross-Linking Reagents (chemistry)
• Disease Models, Animal
• Epitopes (metabolism)
• Female
• Food Handling
• Glycoproteins (metabolism)
• Humans
• Immunization
• Immunoglobulin E (metabolism)
• Mice, Inbred C3H
• Monophenol Monooxygenase (chemistry)
• Peanut Hypersensitivity (immunology)
• Plant Proteins (chemistry, immunology, metabolism, pharmacokinetics)