Overexpression of insulin-like growth factor-I receptor as a pertinent biomarker for hepatocytes malignant transformation.

Abstract

AIM:

To investigate the dynamic features of insulin-like growth factor-I receptor (IGF-IR) expression in rat hepatocarcinogenesis, and the relationship between IGF-IR and hepatocytes malignant transformation at mRNA or protein level.

METHODS:

Hepatoma models were made by inducing with 2-fluorenylacetamide (2-FAA) on male Sprague-Dawley rats. Morphological changes of hepatocytes were observed by pathological Hematoxylin and eosin staining, the dynamic expressions of liver and serum IGF-IR were quantitatively analyzed by an enzyme-linked immunosorbent assay. The distribution of hepatic IGF-IR was located by immunohistochemistry. The fragments of IGF-IR gene were amplified by reverse transcription-polymerase chain reaction, and confirmed by sequencing.

RESULTS:

Rat hepatocytes after induced by 2-FAA were changed dynamically from granule-like degeneration, precancerous to hepatoma formation with the progressing increasing of hepatic mRNA or IGF-IR expression. The incidences of liver IGF-IR, IGF-IR mRNA, specific IGF-IR concentration (ng/mg wet liver), and serum IGF-IR level (ng/mL) were 0.0%, 0.0%, 0.63 ± 0.17, and 1.33 ± 0.47 in the control; 50.0%, 61.1%, 0.65 ± 0.2, and 1.51 ± 0.46 in the degeneration; 88.9%, 100%, 0.66 ± 0.14, and 1.92 ± 0.29 in the precancerosis; and 100%, 100%, 0.96 ± 0.09, and 2.43 ± 0.57 in the cancerous group, respectively. IGF-IR expression in the cancerous group was significantly higher (P < 0.01) than that in any of other groups at mRNA or protein level. The closely positive IGF-IR relationship was found between livers and sera (r = 0.91, t = 14.222, P < 0.01), respectively.

CONCLUSION:

IGF-IR expression may participate in rat hepatocarcinogenesis and its abnormality should be an early marker for hepatocytes malignant transformation.

• Authors: Xiao-Di Yan, Min Yao, Li Wang, Hai-Jian Zhang, Mei-Juan Yan, Xing Gu, Yun Shi, Jie Chen, Zhi-Zhen Dong, Deng-Fu Yao
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 19 Issue 36 Pg. 6084-92 (Sep 28 2013) ISSN: 2219-2840 [Electronic] United States
• PMID: 24106410 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• RNA, Messenger
• 2-Acetylaminofluorene
• Receptor, IGF Type 1

Topics

• 2-Acetylaminofluorene
• Animals
• Biomarkers, Tumor (blood, genetics, metabolism)
• Carcinoma, Hepatocellular (chemically induced, genetics, metabolism, pathology)
• Cell Transformation, Neoplastic (chemically induced, genetics, metabolism, pathology)
• Disease Models, Animal
• Gene Expression Regulation, Neoplastic
• Hepatocytes (metabolism, pathology)
• Liver Neoplasms (chemically induced, genetics, metabolism, pathology)
• Male
• RNA, Messenger (metabolism)
• Rats
• Rats, Sprague-Dawley
• Receptor, IGF Type 1 (blood, genetics, metabolism)
• Up-Regulation