[Protective effect of anhydroicaritin against peritonitis in mice].

Abstract

OBJECTIVE:

To investigate the effect of anhydroicaritin (AHI) against zymosan-induced peritonitis in mice.

METHODS:

Peritonitis was induced in mice by intraperitoneal injection of zymosan. All mice were monitored for systemic toxicity and mortality for 13 d after zymosan or saline administration. In another set of experiments, the peritoneal exudates were collected. The leukocyte numbers and the production of inflammatory cytokines (IL-6, IL-10, TNF-α, MCP-1) were determined by flow cytometry. The release of nitric oxide (NO) was measured by a Griess reagent system. The Ca(2+); influx in bone marrow-derived macrophages was recorded by laser scanning confocal microscopy with Fluo4-AM loading. The expression of iNOS was determined by Western blotting.

RESULTS:

AHI (4 mg/kg) prolonged survival of peritonitis mice, inhibited massive leukocyte transmigration into the peritoneal cavity, and decreased the overproduction of NO, IL-10, TNF-α, MCP-1 and IL-6. In LPS-stimulated mouse macrophages, AHI (5 μmol/L) pretreatment significantly inhibited the elevation of intracellular Ca(2+);, and markedly decreased iNOS protein expression.

CONCLUSION:

AHI possesses significant protective effects on the zymosan-induced peritonitis mice, which might be associated with the regulation of Ca(2+); influx in macrophages and iNOS expression.

• Authors: Xinqiang Lai, Xiuyan Huang, Yaoying Zeng
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 29 Issue 10 Pg. 1036-9 (Oct 2013) ISSN: 1007-8738 [Print] China
• PMID: 24103264 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzopyrans
• Cytokines
• anhydroicaritin
• Nitric Oxide Synthase Type II
• Calcium

Topics

• Animals
• Benzopyrans (therapeutic use)
• Calcium (metabolism)
• Cytokines (analysis)
• Female
• Leukocyte Count
• Macrophages (metabolism)
• Mice
• Mice, Inbred BALB C
• Nitric Oxide Synthase Type II (analysis)
• Peritonitis (prevention & control)