The cytotoxic effect of α-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction.
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| Abstract |
In recent years, α-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of α-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 µM but then a recovery of cells occurred. The recovery was not caused by the biotransformation of α-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of α-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of α-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in α-tomatine-treated cells. These results support the conclusion that α-tomatine does not induce apoptosis in the MCF-7 cell line.
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| Authors | Lenka Sucha, Milos Hroch, Martina Rezacova, Emil Rudolf, Radim Havelek, Ludek Sispera, Jana Cmielova, Renata Kohlerova, Ales Bezrouk, Pavel Tomsik |
| Journal | Oncology reports (Oncol Rep) Vol. 30 Issue 6 Pg. 2593-602 (Dec 2013) ISSN: 1791-2431 [Electronic] Greece |
| PMID | 24100733 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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