Inhibition of human T-cell lymphotropic virus type III in vitro by phosphonoformate.

Abstract

Phosphonoformate, an inhibitor of reverse transcriptase in a number of retroviruses, was shown to have a dose-related inhibitory effect on human T-cell lymphotropic virus type III (HTLV-III) replication in the H9 cell line in vitro. HTLV-III replication was eliminated at a concentration of 680 mumol, a non-cytotoxic dose. A lower dose of 132 mumol inhibited HTLV-III replication by more than 98%, as measured by reverse transcriptase activity, compared with untreated infected cultures. Reverse transcriptase activity in HTLV-III particles was completely inhibited by 5.0 mumol phosphonoformate.

• Authors: E G Sandstrom, J C Kaplan, R E Byington, M S Hirsch
• Journal: Lancet (London, England) (Lancet) Vol. 1 Issue 8444 Pg. 1480-2 (Jun 29 1985) ISSN: 0140-6736 [Print] England
• PMID: 2409414 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Organophosphorus Compounds
• Foscarnet
• RNA-Directed DNA Polymerase
• Phosphonoacetic Acid

Topics

• Cell Line
• Deltaretrovirus (drug effects, enzymology, growth & development)
• Dose-Response Relationship, Drug
• Foscarnet
• Humans
• Organophosphorus Compounds (pharmacology)
• Phosphonoacetic Acid (analogs & derivatives, pharmacology)
• RNA-Directed DNA Polymerase (metabolism)
• Time Factors
• Virus Replication (drug effects)