Mucosal vaccination with recombinant adenovirus encoding nucleoprotein provides potent protection against influenza virus infection.

Abstract

Influenza vaccines that target the highly variable surface glycoproteins hemagglutinin and neuraminidase cause inconvenience of having vaccination every year. For this reason, development of universal vaccines targeting conserved viral components is needed. In this study, we generated recombinant adenovirus (rAd) vaccine encoding nucleoprotein (NP) of A/PR/8/34 influenza virus, designated rAd/NP. BALB/c mice were immunized intranasally or sublingually with rAd/NP vaccine and subsequently challenged with lethal doses of heterologous as well as homologous influenza viruses. We found that intranasal immunization of rAd/NP elicited strong mucosal IgA responses as well as stronger CD8 T-cell responses toward immunodominant K(d)-restricted NP147-155 epitope than sublingual immunization. Importantly, only single intranasal but not sublingual immunization of rAd/NP provides potent protection against both homologous and heterologous influenza virus challenges. These results suggest that recombinant rAd/NP could be a universal vaccine candidate for mucosal administration against influenza virus.

• Authors: So-Hee Kim, Joo Young Kim, Youngjoo Choi, Huan H Nguyen, Man Ki Song, Jun Chang
• Journal: PloS one (PLoS One) Vol. 8 Issue 9 Pg. e75460 ( 2013) ISSN: 1932-6203 [Electronic] United States
• PMID: 24086536 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• Immunoglobulin A
• Influenza Vaccines
• Nucleoproteins
• Vaccines, Synthetic

Topics

• Adenoviridae (genetics, metabolism)
• Administration, Mucosal
• Animals
• Bronchoalveolar Lavage Fluid (immunology)
• CD8-Positive T-Lymphocytes (immunology)
• DNA Primers (genetics)
• Enzyme-Linked Immunosorbent Assay
• HEK293 Cells
• Humans
• Immunoglobulin A (immunology)
• Influenza Vaccines (administration & dosage, metabolism, therapeutic use)
• Influenza, Human (prevention & control)
• Mice
• Mice, Inbred BALB C
• Nucleoproteins (metabolism)
• Vaccines, Synthetic (administration & dosage, metabolism, therapeutic use)