Abstract |
In the previous study, the anti-inflammatory effect of p-cymene had been found. In this study, we investigated anti-inflammatory effects of p-cymene on acute lung injury using lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators including tumor necrosis factor alpha (TNF-α), IL-1β, and IL-6 were assayed by enzyme-linked immunosorbent assay method. The pathological changes of the lung tissues were observed by hematoxylin and eosin staining. The inflammatory signal pathway-related protein levels of NF-κB were measured using Western blotting. The data showed that treatment with the p-cymene markedly attenuated inflammatory cell numbers in the BALF, decreased NF-κB protein level in the lungs, improved SOD activity, and inhibited MPO activity. Histological studies demonstrated that p-cymene substantially inhibited LPS-induced neutrophils in the lung tissue compared with the model group. The results indicated that p-cymene had a protective effect on LPS-induced ALI in mice.
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Authors | Lvyi Chen, Lei Zhao, Chunfeng Zhang, Zhou Lan |
Journal | Inflammation
(Inflammation)
Vol. 37
Issue 2
Pg. 358-64
(Apr 2014)
ISSN: 1573-2576 [Electronic] United States |
PMID | 24085645
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents
- Cymenes
- Inflammation Mediators
- Interleukin-1beta
- Interleukin-6
- Lipopolysaccharides
- Monoterpenes
- Rela protein, mouse
- Transcription Factor RelA
- Tumor Necrosis Factor-alpha
- interleukin-6, mouse
- 4-cymene
- Peroxidase
- Superoxide Dismutase
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Topics |
- Acute Lung Injury
(chemically induced, immunology, metabolism, prevention & control)
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Bronchoalveolar Lavage Fluid
(immunology)
- Cymenes
- Cytoprotection
- Disease Models, Animal
- Female
- Inflammation Mediators
(metabolism)
- Interleukin-1beta
(metabolism)
- Interleukin-6
(metabolism)
- Lipopolysaccharides
- Lung
(drug effects, immunology, metabolism)
- Mice
- Mice, Inbred BALB C
- Monoterpenes
(pharmacology)
- Neutrophil Infiltration
(drug effects)
- Peroxidase
(metabolism)
- Pulmonary Edema
(chemically induced, prevention & control)
- Superoxide Dismutase
(metabolism)
- Transcription Factor RelA
(metabolism)
- Tumor Necrosis Factor-alpha
(metabolism)
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