Antioxidative capacity in the fat body of Bombyx mori is increased following oral administration of 4-methylumbelliferone.

Abstract

Plant sources of umbelliferones have tumor-inhibitory effects at the cellular level. However, their physiological functions in animals are largely unresolved. In this study, we provide evidence to show that 4-methylumbelliferone (4-MU) participates in the regulation of antioxidative capacity in the fat body of Bombyx mori, a tissue similar to mammalian liver in this model invertebrate. Larvae (3rd day of the 5th instar) were orally exposed to 4 mM 4-MU, an umbelliferone, which swiftly induced the generation of a large number of ROS (e.g. H2O2 increased 6 to 8-fold), and 4-MU was detected in the fat body 8 min after administration. In addition, the activities of CAT and GPx were up-regulated 4 to 11-fold and 2 to 16-fold, respectively, and were helpful in defending fat body cells against oxidative injury in combination with NADPH. Furthermore, significant increases in the contents of T-AOC (up to approx. 2-fold), antioxidants of ASAFR (by 2 to 4-fold) and GSH were detected.

• Authors: Yan Fang, Hua Wang, Wenjuan Zhu, Lu Wang, Hengjiang Liu, Yue He, Xu Xu, Weimin Yin, Yanghu Sima, Shiqing Xu
• Journal: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP (Comp Biochem Physiol C Toxicol Pharmacol) Vol. 159 Pg. 31-7 (Jan 2014) ISSN: 1532-0456 [Print] United States
• PMID: 24080584 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013 Elsevier Inc. All rights reserved.

Chemical References

• Antioxidants
• Reactive Oxygen Species
• Hymecromone
• NADP
• Hydrogen Peroxide
• Catalase
• Glutathione Peroxidase
• Glutathione Reductase
• Glutathione

Topics

• Animals
• Antioxidants (metabolism)
• Bombyx (drug effects, metabolism)
• Catalase (metabolism)
• Fat Body (drug effects, metabolism)
• Glutathione (metabolism)
• Glutathione Peroxidase (metabolism)
• Glutathione Reductase (metabolism)
• Hydrogen Peroxide (metabolism)
• Hymecromone (pharmacology)
• Larva (drug effects, metabolism)
• NADP (metabolism)
• Oxidative Stress (drug effects)
• Reactive Oxygen Species (metabolism)