[Study on effect of weinaokang and bilobalide on autophagy and neurogenesis induced by focal cerebral ischemia reperfusion].

Abstract

OBJECTIVE:

To observe the protective effect of the Weinaokang (WNK) and its active compound bilobalide on focal cerebral ischemia reperfusion, and their mechanisms.

METHOD:

The 60-minute middle cerebral artery occlusion (MCAO) was adopted to establish the 24 h-14 d reperfusion model. The expression of Beclin-1 was detected by the Western blotting technique. The transmission electron microscopy was used to observe ultrastructural changes. Neurogenesis was detected by the immunofluorescence staining.

RESULT:

WNK (20, 10 mg x kg(-1), ig) or its active compound bilobalide (10, 5 mg x kg(-1), ig) could promote the generation of mature neurons (BrdU(+) -MAP-2+) at the ischemic side, and inhibit expression of autophagy-related gene Beclin-1, so as to reduce the neuron injury induced by focal cerebral ischemia reperfusion.

CONCLUSION:

WNK and its active compound bilobalide can inhibit neuron autophagy and improve neurogenesis in ischemic peripheral area, suggesting that neurogenesis may be the intervention target for WNK to promote self-repairing of ischemic area.

• Authors: Yong-Qiu Zheng, Jian-Xun Liu, Li Xu, Ming-Jiang Yao, Wen-Ting Song
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 38 Issue 13 Pg. 2182-6 (Jul 2013) ISSN: 1001-5302 [Print] China
• PMID: 24079250 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyclopentanes
• Drugs, Chinese Herbal
• Furans
• Ginkgolides
• weinaokang
• bilobalide

Topics

• Animals
• Autophagy (drug effects)
• Brain Ischemia (drug therapy, pathology, physiopathology)
• Cyclopentanes (pharmacology)
• Drugs, Chinese Herbal (pharmacology)
• Furans (pharmacology)
• Ginkgolides (pharmacology)
• Male
• Neurogenesis (drug effects)
• Neurons (ultrastructure)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (prevention & control)