Production of 2-butanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain.

Abstract

Klebsiella pneumoniae was engineered to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, ∆ldhA/pBR-iBO (ilvIH–ilvC–ilvD–kivd–adhA), produced 2-butanol (160 mg l−1) from crude glycerol. To increase the yield of 2-butanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of 2-butanol from 160 to 320 mg l−1. This represents the first successful attempt to produce 2-butanol from crude glycerol.

• Authors: Baek-Rock Oh, Sun-Yeon Heo, Sung-Mok Lee, Won-Kyung Hong, Jang Min Park, You Ree Jung, Dae-Hyuk Kim, Jung-Hoon Sohn, Jeong-Woo Seo, Chul Ho Kim
• Journal: Biotechnology letters (Biotechnol Lett) Vol. 36 Issue 1 Pg. 57-62 (Jan 2014) ISSN: 1573-6776 [Electronic] Netherlands
• PMID: 24078128 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Butanols
• 2-butanol
• Glycerol

Topics

• Bioreactors
• Butanols (analysis, metabolism)
• Fermentation
• Genetic Engineering
• Glycerol (metabolism)
• Klebsiella pneumoniae (genetics, metabolism)