[Effect of the C-terminal truncated human apoptosis-inducing factor δ1-480 on biological behaviors of MCF-7 cells].

Abstract

OBJECTIVE:

To observe the expression of the C-terminal truncated human apoptosis-inducing factor (AIF) and its biological effect on MCF-7 cells.

METHODS:

PcDNA3.0-FDT-AIFδ1-480 was transfected into human breast carcinoma MCF-7 cells with lipofectamine. The expression of the truncated AIF gene was detected by Western blotting, and its effects on the biological behaviors of MCF-7 cells and on the expression of cytochrome c (cytC) were evaluated using flow cytometry, MTT assay, colony-forming assay, and mitochondrial membrane potential measurement.

RESULTS:

PcDNA3.0-FDT-AIFδ1-480 enhanced AIF expression in MCF-7 cells, obviously inhibited the cell proliferation, and significantly reduced the mitochondrial membrane potentials (P<0.05). Transfection of the cells with PcDNA3.0-FDT-AIFδ1-480 promoted the expression of cytC and resulted in significantly increased apoptosis of MCF-7 cells (P<0.05).

CONCLUSION:

The expression of C-terminal truncated human AIF gene can induce apoptosis of human MCF-7 cells by promoting cytC release from mitochondria.

• Authors: Lijuan Wang, Huawei Xiao, E Bai, Xia Zhou, Meng Li, Guihua Jin, Qing Zhu
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 33 Issue 9 Pg. 1285-8 (Sep 2013) ISSN: 1673-4254 [Print] China
• PMID: 24067204 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AIFM1 protein, human
• Apoptosis Inducing Factor
• Cytochromes c

Topics

• Apoptosis
• Apoptosis Inducing Factor (genetics, metabolism)
• Breast Neoplasms (metabolism, pathology)
• Cell Proliferation
• Cytochromes c (genetics, metabolism)
• Female
• Humans
• MCF-7 Cells
• Membrane Potential, Mitochondrial
• Mitochondria (metabolism)