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Influenza virosomes supplemented with GPI-0100 adjuvant: a potent vaccine formulation for antigen dose sparing.

Abstract
Adjuvants can stimulate vaccine-induced immune responses and can contribute decisively to antigen dose sparing when vaccine antigen production is limited, as for example during a pandemic influenza outbreak. We earlier showed that GPI-0100, a semi-synthetic saponin derivative with amphiphilic structure, significantly stimulates the immunogenicity and protective efficacy of influenza subunit vaccine administered via a systemic route. Here, we evaluated the adjuvant effect of GPI-0100 on a virosomal influenza vaccine formulation. In contrast to influenza subunit vaccine adjuvanted with GPI-0100, virosomal vaccine supplemented with the same dose of GPI-0100 provided full protection of mice against infection at the extremely low antigen dose of 2 × 8 ng hemagglutinin. Overall, adjuvanted virosomes elicited higher antibody and T-cell responses than did adjuvanted subunit vaccine. The enhanced immunogenicity of the GPI-0100-adjuvanted virosomes, particularly at low antigen doses, is possibly due to a physical association of the amphiphilic adjuvant with the virosomal membrane. These results show that a combination of GPI-0100 and a virosomal influenza vaccine formulation is highly immunogenic and allows the use of very low antigen doses without compromising the protective potential of the vaccine.
AuthorsHeng Liu, Jacqueline de Vries-Idema, Wouter Ter Veer, Jan Wilschut, Anke Huckriede
JournalMedical microbiology and immunology (Med Microbiol Immunol) Vol. 203 Issue 1 Pg. 47-55 (Feb 2014) ISSN: 1432-1831 [Electronic] Germany
PMID24062182 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adjuvants, Immunologic
  • Antigens, Viral
  • GPI0100
  • Influenza Vaccines
  • Saponins
  • Vaccines, Subunit
  • Vaccines, Virosome
Topics
  • Adjuvants, Immunologic
  • Animals
  • Antigens, Viral (immunology)
  • Cell Line
  • Disease Models, Animal
  • Dogs
  • Female
  • Immunity, Cellular
  • Immunity, Humoral
  • Influenza A Virus, H1N1 Subtype (immunology)
  • Influenza Vaccines (administration & dosage, immunology)
  • Mice
  • Orthomyxoviridae Infections (prevention & control)
  • Saponins (administration & dosage, immunology)
  • Vaccines, Subunit
  • Vaccines, Virosome

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