Abstract |
Adjuvants can stimulate vaccine-induced immune responses and can contribute decisively to antigen dose sparing when vaccine antigen production is limited, as for example during a pandemic influenza outbreak. We earlier showed that GPI-0100, a semi-synthetic saponin derivative with amphiphilic structure, significantly stimulates the immunogenicity and protective efficacy of influenza subunit vaccine administered via a systemic route. Here, we evaluated the adjuvant effect of GPI-0100 on a virosomal influenza vaccine formulation. In contrast to influenza subunit vaccine adjuvanted with GPI-0100, virosomal vaccine supplemented with the same dose of GPI-0100 provided full protection of mice against infection at the extremely low antigen dose of 2 × 8 ng hemagglutinin. Overall, adjuvanted virosomes elicited higher antibody and T-cell responses than did adjuvanted subunit vaccine. The enhanced immunogenicity of the GPI-0100-adjuvanted virosomes, particularly at low antigen doses, is possibly due to a physical association of the amphiphilic adjuvant with the virosomal membrane. These results show that a combination of GPI-0100 and a virosomal influenza vaccine formulation is highly immunogenic and allows the use of very low antigen doses without compromising the protective potential of the vaccine.
|
Authors | Heng Liu, Jacqueline de Vries-Idema, Wouter Ter Veer, Jan Wilschut, Anke Huckriede |
Journal | Medical microbiology and immunology
(Med Microbiol Immunol)
Vol. 203
Issue 1
Pg. 47-55
(Feb 2014)
ISSN: 1432-1831 [Electronic] Germany |
PMID | 24062182
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Adjuvants, Immunologic
- Antigens, Viral
- GPI0100
- Influenza Vaccines
- Saponins
- Vaccines, Subunit
- Vaccines, Virosome
|
Topics |
- Adjuvants, Immunologic
- Animals
- Antigens, Viral
(immunology)
- Cell Line
- Disease Models, Animal
- Dogs
- Female
- Immunity, Cellular
- Immunity, Humoral
- Influenza A Virus, H1N1 Subtype
(immunology)
- Influenza Vaccines
(administration & dosage, immunology)
- Mice
- Orthomyxoviridae Infections
(prevention & control)
- Saponins
(administration & dosage, immunology)
- Vaccines, Subunit
- Vaccines, Virosome
|