Rapid differentiation and quantification of live/dead cancer cells using differential photochemical behavior of acridine orange.

Abstract

This paper demonstrates for the first time a simple analytical method for differentiation and quantification of dead/live cancer cells using acridine orange (AO) enabled fluorescence spectroscopic techniques. Based on the differential fluorescence (live cells fluoresce green and dead cells orange) exhibited when intercalated with AO, the live/dead cells can be easily differentiated. The optimal AO concentration for enhanced sensitive differentiation has been optimized as 0.001%. These studies offer a promising application for rapid differentiation and quantification of live/dead cells in the case of cytotoxic treatment/therapies within several minutes.

• Authors: M Manikandan, Hui-Fen Wu
• Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology (Photochem Photobiol Sci) Vol. 12 Issue 11 Pg. 1921-6 (Nov 2013) ISSN: 1474-9092 [Electronic] England
• PMID: 24057226 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acridine Orange

Topics

• Acridine Orange (chemistry, radiation effects)
• Animals
• Cell Death
• Cell Differentiation
• Cell Survival
• Mice
• Neuroblastoma (pathology)
• Photochemical Processes
• Spectrometry, Fluorescence
• Tumor Cells, Cultured