Abstract |
Vasonatrin peptide (VNP), a novel manmade natriuretic peptide, is known as a cardiovascular active substance. However, its neuroeffects are largely unknown. Here, cultured dopaminergic neurons from ventral mesencephalon of mouse were exposed to N-methyl-4-phenylpyridinium (MPP(+)), and the effects of VNP on the neurotoxicity of MPP(+) were investigated. As a result, MPP(+) caused injuries in the dopaminergic neurons. VNP significantly reduced the cytotoxicity of MPP(+) by increasing axon number and length of dopaminergic neurons, and by enhancing the cell viability. Also, the MPP(+)-induced depolymerization of β- Tubulin III was attenuated by the treatment of VNP. In addition, VNP significantly increased the intracellular levels of cGMP. These effects of VNP were mimicked by 8-br-cGMP (a cell-permeable analog of cGMP), whereas inhibited by HS-142-1 (the antagonist of the particulate guanylyl cyclase-coupled natriuretic peptide receptors), or KT-5823 (a cGMP-dependent protein kinase inhibitor). Taken together, VNP attenuates the neurotoxicity of MPP(+) via guanylyl cyclase-coupled NPR/cGMP/PKG pathway, indicating that VNP might be a new effective reagent in the treatment of neuron degeneration of dopaminergic neurons in Parkinson's disease (PD).
|
Authors | Hai-Kang Zhao, Bao-Ying Chen, Rui Chang, Jian-Bang Wang, Feng Ni, Lei Yang, Xi-Chao Dong, Shu-Hu Sun, Ge Zhao, Wei Fang, Quan-Rui Ma, Xue-Lian Wang, Jun Yu |
Journal | Peptides
(Peptides)
Vol. 49
Pg. 117-22
(Nov 2013)
ISSN: 1873-5169 [Electronic] United States |
PMID | 24055805
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2013 Elsevier Inc. All rights reserved. |
Chemical References |
- Neuroprotective Agents
- ventricular natriuretic peptide, eel
- Atrial Natriuretic Factor
- 1-Methyl-4-phenylpyridinium
- Dopamine
|
Topics |
- 1-Methyl-4-phenylpyridinium
(toxicity)
- Animals
- Atrial Natriuretic Factor
(pharmacology)
- Dopamine
(metabolism)
- Mice
- Mice, Inbred C57BL
- Neurons
(drug effects, metabolism)
- Neuroprotective Agents
(toxicity)
|