Tissue tropism of Ornithobacterium rhinotracheale in chickens determined by culture and nucleic acid detection.

The aim of the present study was to evaluate the ability of Ornithobacterium rhinotracheale (ORT) to colonize chosen organs of chicks infected intratracheally (group A1), or intravenously (group A2), with the use of bacteriological methods and PCR. The bacteriological methods enabled to reisolate ORT bacteria from trachea and lungs of the birds from group A1 only on day 3 and 6 after infection. The PCR technique additionally detected the bacterial genetic material in these organs on the 9th day after infection, and gave positive results in the samples from air sacs until the 6th day of the experiment. In birds infected intravenously (A2) ORT was reisolated from liver on day 3 and from spleen on day 3 and 6 after infection, whereas the reisolation from the tibiotarsal joint occurred during the entire experimental period. PCR enabled to detect the bacterial DNA in the liver, spleen and lungs of chickens until the 9th day after infection and in case of tibiotarsal joint during the whole time of the study.
AuthorsArtur Zbikowski, Piotr Szeleszczuk, Marian Binek
JournalPolish journal of microbiology / Polskie Towarzystwo MikrobiologoĢw = The Polish Society of Microbiologists (Pol J Microbiol) Vol. 62 Issue 2 Pg. 205-9 ( 2013) ISSN: 1733-1331 [Print] Poland
PMID24053025 (Publication Type: Journal Article)
Chemical References
  • DNA, Bacterial
  • Animals
  • Chickens
  • DNA, Bacterial (genetics)
  • Flavobacteriaceae Infections (microbiology, pathology, veterinary)
  • Hindlimb
  • Joints (microbiology)
  • Liver (microbiology)
  • Organ Specificity
  • Ornithobacterium (isolation & purification)
  • Polymerase Chain Reaction (veterinary)
  • Respiratory System (microbiology)
  • Spleen (microbiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: