Adenovirus-based vaccine against Listeria monocytogenes: extending the concept of invariant chain linkage.

The use of replication-deficient adenoviruses as vehicles for transfer of foreign genes offers many advantages in a vaccine setting, eliciting strong cellular immune responses involving both CD8(+) and CD4(+) T cells. Further improving the immunogenicity, tethering of the inserted target Ag to MHC class II-associated invariant chain (Ii) greatly enhances both the presentation of most target Ags, as well as overall protection against viral infection, such as lymphocytic choriomeningitis virus (LCMV). The present study extends this vaccination concept to include protection against intracellular bacteria, using Listeria monocytogenes as a model organism. Protection in C57BL/6 mice against recombinant L. monocytogenes expressing an immunodominant epitope of the LCMV glycoprotein (GP33) was greatly accelerated, augmented, and prolonged following vaccination with an adenoviral vaccine encoding GP linked to Ii compared with vaccination with the unlinked vaccine. Studies using knockout mice demonstrated that CD8(+) T cells were largely responsible for this protection, which is mediated through perforin-dependent lysis of infected cells and IFN-γ production. Taking the concept a step further, vaccination of C57BL/6 (L. monocytogenes-resistant) and BALB/c (L. monocytogenes-susceptible) mice with adenoviral vectors encoding natural L. monocytogenes-derived soluble Ags (listeriolysin O and p60) revealed that tethering of these Ags to Ii markedly improved the vaccine-induced CD8(+) T cell response to two of three epitopes studied. More importantly, Ii linkage accelerated and augmented vaccine-induced protection in both mouse strains and prolonged protection, in particular that induced by the weak Ag, p60, in L. monocytogenes-susceptible BALB/c mice.
AuthorsSøren Jensen, Maria Abildgaard Steffensen, Benjamin Anderschou Holbech Jensen, Dirk Schlüter, Jan Pravsgaard Christensen, Allan Randrup Thomsen
JournalJournal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 191 Issue 8 Pg. 4152-64 (Oct 15 2013) ISSN: 1550-6606 [Electronic] United States
PMID24043891 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Differentiation, B-Lymphocyte
  • Antigens, Viral
  • Bacterial Proteins
  • Bacterial Toxins
  • Bacterial Vaccines
  • Epitopes, T-Lymphocyte
  • Glycoproteins
  • Heat-Shock Proteins
  • Hemolysin Proteins
  • Histocompatibility Antigens Class II
  • Lipoproteins
  • O Antigens
  • P60 protein, bacteria
  • Peptide Fragments
  • Viral Proteins
  • glycoprotein peptide 33-41, Lymphocytic choriomeningitis virus
  • invariant chain
  • Perforin
  • hlyA protein, Listeria monocytogenes
  • Interferon-gamma
  • Adenoviridae (genetics)
  • Animals
  • Antigens, Differentiation, B-Lymphocyte (immunology)
  • Antigens, Viral (genetics, immunology)
  • Bacterial Proteins (genetics, immunology)
  • Bacterial Toxins (genetics, immunology)
  • Bacterial Vaccines
  • Base Sequence
  • CD8-Positive T-Lymphocytes (immunology)
  • Epitopes, T-Lymphocyte (genetics, immunology)
  • Female
  • Genetic Vectors
  • Glycoproteins (genetics, immunology)
  • Heat-Shock Proteins (genetics, immunology)
  • Hemolysin Proteins (genetics, immunology)
  • Histocompatibility Antigens Class II (immunology)
  • Immunity, Cellular
  • Interferon-gamma (biosynthesis, immunology)
  • Lipoproteins (genetics, immunology)
  • Listeria monocytogenes (immunology)
  • Listeriosis (immunology, prevention & control)
  • Lymphocytic Choriomeningitis (immunology, prevention & control)
  • Lymphocytic choriomeningitis virus (genetics, immunology)
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Molecular Sequence Data
  • O Antigens (genetics, immunology)
  • Peptide Fragments (genetics, immunology)
  • Perforin (immunology)
  • Viral Proteins (genetics, immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: