miR-203 inhibits cell proliferation and migration of lung cancer cells by targeting PKCα.

Abstract

PKCα (protein kinase C alpha, PRKCA) is an important protein involved in several steps of signaling pathways in lung cancer, and microRNAs (miRNAs) have also been shown to participate in lung carcinogenesis. However, it is not clear how PKCα and miRNAs are correlated in the disease. In this report, we aimed to identify novel miRNAs that target PKCα and to study their biological function. Using bioinformatics analysis, we predicted one novel candidate, miR-203, and found differential expression patterns of miR-203 and PKCα in human lung cancer tissues. Moreover, we experimentally validated miR-203 as a direct regulator of PKCα. Finally, we demonstrated that the targeting of PKCα by miR-203 played a critical role in regulating cell proliferation, apoptosis and migration in lung cancer cells. In summary, this study identifies a novel miRNA that targets PKCα and illustrates that the downregulation of PKCα by miR-203 modulates biological processes in lung cancer cells.

• Authors: Chen Wang, Xueliang Wang, Hongwei Liang, Tao Wang, Xin Yan, Minghui Cao, Nan Wang, Suyang Zhang, Ke Zen, Chenyu Zhang, Xi Chen
• Journal: PloS one (PLoS One) Vol. 8 Issue 9 Pg. e73985 ( 2013) ISSN: 1932-6203 [Electronic] United States
• PMID: 24040137 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 3' Untranslated Regions
• MIRN203 microRNA, human
• MicroRNAs
• Protein Kinase C-alpha

Topics

• 3' Untranslated Regions
• Aged
• Apoptosis (genetics)
• Base Pairing
• Base Sequence
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms (genetics, metabolism, pathology)
• Male
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Neoplasm Staging
• Protein Kinase C-alpha (genetics, metabolism)
• Reproducibility of Results