Lipophilic amino alcohols reduces carrageenan-induced paw edema and anti-OVA DTH in BALB/c mice.

Abstract

The inflammation process is a coordinated response of the organism related to immune response with release of pro-inflammatory substances, as nitric oxide, TNF-α and IL-1β. In this work, a series of lipophilic amino alcohols were evaluated on RAW264.7 and primary macrophages for the modulation of nitric oxide and TNF-α. The most potent compounds were submitted to the treatment of BALB/c mice and evaluation of the carrageenan-induced paw edema and TNF-α and IL1-β release in the paws and anti-OVA delayed type hypersensitivity reaction. RAW264.7 and primary macrophages were incubated in the presence of amino alcohols at different concentrations (1, 0.5, 0.05 and 0.005 μg mL(-1)). All tested compounds were not cytotoxic, however the inhibition of NO and TNF-α were observed only in RAW264.7 cultures. The NO production were reduced in 100% for all compounds, but only the compounds 4a and 4b expressively reduced the TNF-α release (67% and 92% respectively). On the carrageenan-induced paw edema, the compound 4b treatment showed reduction of edema, TNF-α and IL-1β as efficient as dexamethasone treatment. Meanwhile, the compound 4a treatment showed only slight reduction of paw edema. In the anti-OVA DTH reaction, both compounds showed reduction in the paw edema as effective as dexamethasone. In function of the observed results in vitro and in the acute and anti-OVA inflammation of mice paw edema compound 4b showed promissory anti-inflammatory properties.

• Authors: Elaine F C Reis, Sandra B R Castro, Caio Cesar S Alves, Erick E Oliveira, Tais A Corrêa, Mauro V Almeida, Ana Paula Ferreira
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 17 Issue 3 Pg. 727-32 (Nov 2013) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 24035232 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2013.

Chemical References

• Allergens
• Amino Alcohols
• Anti-Inflammatory Agents
• Interleukin-1beta
• Lipopolysaccharides
• Tumor Necrosis Factor-alpha
• Nitric Oxide
• Carrageenan
• Ovalbumin

Topics

• Allergens
• Amino Alcohols (pharmacology, therapeutic use)
• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Carrageenan
• Cell Line
• Cells, Cultured
• Edema (chemically induced, drug therapy, immunology)
• Foot
• Hypersensitivity, Delayed (drug therapy, immunology)
• Interleukin-1beta (immunology)
• Lipopolysaccharides
• Macrophages (drug effects, immunology)
• Male
• Mice
• Mice, Inbred BALB C
• Nitric Oxide (metabolism)
• Ovalbumin
• Tumor Necrosis Factor-alpha (immunology)