Induction of apoptosis and inhibition of invasion in choriocarcinoma JEG-3 cells by α-calendic acid and β-calendic acid.

Abstract

Alfa-calendic acid and β-calendic acid, geometric and positional isomers of linolenic acid were previously shown to possess potent anticancer properties. In this study, we found that α-calendic acid and β-calendic acid could induce apoptosis and suppress invasion of human choriocarcinoma JEG-3 cells in vitro. Treatment with α-calendic acid and β-calendic acid significantly increased oxidative stress in human choriocarcinoma JEG-3 cells detected by the level of reactive oxygen species (ROS), lipid peroxidation production malondialdehyde (MDA), glutathione (GSH) and the effects of antioxidants NAC and α-tocopherol. Furthermore, oxidative stress activated the phosphorylation of p38MAPK. SB203580, a selective p38MAPK inhibitor, blocked the apoptosis induced by α-calendic acid and β-calendic acid by upregulating Bcl-2/Bax ratio and inhibition of the activation of Caspase-3 and Caspase-9. SB20350 also partially abrogated the cell invasion effects of α-calendic acid and β-calendic acid. These results suggested that α-calendic acid and β-calendic acid induced apoptosis and inhibited invasion in JEG-3 cells by activation of oxidative stress pathways and subsequent activation of P38MAPK.

• Authors: Qian Li, Han Wang, Shuhong Ye, Shan Xiao, Yuping Xie, Xiao Liu, Jihui Wang
• Journal: Prostaglandins, leukotrienes, and essential fatty acids (Prostaglandins Leukot Essent Fatty Acids) Vol. 89 Issue 5 Pg. 367-76 (Oct 2013) ISSN: 1532-2823 [Electronic] Scotland
• PMID: 24035100 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013. Published by Elsevier Ltd.

Chemical References

• Antineoplastic Agents, Phytogenic
• BAX protein, human
• Fatty Acids, Unsaturated
• Imidazoles
• Pyridines
• Reactive Oxygen Species
• bcl-2 Homologous Antagonist-Killer Protein
• bcl-2-Associated X Protein
• Malondialdehyde
• calendic acid
• p38 Mitogen-Activated Protein Kinases
• Caspases
• Glutathione
• alpha-Tocopherol
• SB 203580
• Acetylcysteine

Topics

• Acetylcysteine (pharmacology)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Caspases (genetics, metabolism)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Fatty Acids, Unsaturated (pharmacology)
• Female
• Gene Expression Regulation, Neoplastic (drug effects)
• Glutathione (metabolism)
• Humans
• Imidazoles (pharmacology)
• Lipid Peroxidation (drug effects)
• Malondialdehyde (metabolism)
• Oxidative Stress
• Pyridines (pharmacology)
• Reactive Oxygen Species (agonists, metabolism)
• Signal Transduction
• Stereoisomerism
• alpha-Tocopherol (pharmacology)
• bcl-2 Homologous Antagonist-Killer Protein (genetics, metabolism)
• bcl-2-Associated X Protein (genetics, metabolism)
• p38 Mitogen-Activated Protein Kinases (genetics, metabolism)