Abstract | OBJECTIVE: METHODS: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction. RESULTS:
DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG gene, 19.8% within the mabA-inhA promoter, and 98.0% at three hotspots within rpoB. Multiplex allele specific polymerase chain reaction detected each of these five mutations, yielding 82.3% sensitivity and 100% specificity for isoniazid resistance, and 97.9% sensitivity and 100% specificity for rifampin resistance as compared to drug susceptibility testing. CONCLUSIONS: The results show that multiplex allele specific polymerase chain reaction is an inexpensive and practical method for rapid detection of multidrug-resistant tuberculosis in developing countries.
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Authors | Xia Wang, Junhua Jiao, Weihua Xu, Xiaoyan Chai, Zhenyun Li, Qingjiang Wang |
Journal | The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases
(Braz J Infect Dis)
2013 Nov-Dec
Vol. 17
Issue 6
Pg. 667-71
ISSN: 1678-4391 [Electronic] Brazil |
PMID | 24029439
(Publication Type: Journal Article)
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Copyright | Copyright © 2013 Elsevier Editora Ltda. All rights reserved. |
Chemical References |
- Antitubercular Agents
- DNA, Bacterial
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Topics |
- Antitubercular Agents
(pharmacology)
- DNA, Bacterial
(analysis)
- Humans
- Microbial Sensitivity Tests
- Molecular Diagnostic Techniques
- Multiplex Polymerase Chain Reaction
(economics)
- Mycobacterium tuberculosis
(drug effects, genetics)
- Point Mutation
- Sensitivity and Specificity
- Sequence Analysis, DNA
- Tuberculosis, Multidrug-Resistant
(diagnosis, microbiology)
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