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[Escherichia coli Nissle 1917 as safe vehicles for intestinal immune targeted therapy--a review].

Abstract
It is difficult to stimulate efficient gut mucosal immune response to intestinal infection. This article critically reviews the research progressin Escherichia coli strain Nisslel917 ( EcN) actingas a safe vehicle for the intestinal mucosal immunity, to restore gastrointestinal disorder and relieve ulcerative colitis. EcN is an orally administered probiotics, combining the excellent colonization and non-immunogenic character, and can be an ideal live vector candidate. This strain could be a tumor-targeted delivery of TAT-Apoptin fusion gene to colorectal cancer. In the treatment of ulcerative colitis and Crohn's disease, the recombinant strain of EcN can be used as a target therapeutics for defensins presenting. Genetically modified EcN could be an ideal carrier organism for gut-focused in situ synthesis and expression of specific localized antigen delivery into the intestine, and stimulate specific mucosal immune response. In vitro trial demonstrated that intestinal recombinant E. coli Nissle-HA110-120 has the potential to stimulate antigen specific response, but EcN itself does not induce mucosal immune response and influence peripheral tolerance to self-antigen. At the same time, there are evidences that EcN is safe. Recombinant E. coli Nissle-HA110-120 does not migrate, clonally expand and activate specific CD4+ T cells, neither in healthy mice nor in other animals with acute colitis, even when the intestinal epithelium suffer from inflammation and the barrier function of the epithelial layer being destroyed.
AuthorsPengpeng Xia, Jun Zhu, Guoqiang Zhu
JournalWei sheng wu xue bao = Acta microbiologica Sinica (Wei Sheng Wu Xue Bao) Vol. 53 Issue 6 Pg. 538-44 (Jun 04 2013) ISSN: 0001-6209 [Print] China
PMID24028055 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Review)
Topics
  • Animals
  • Escherichia coli (genetics, metabolism)
  • Genetic Therapy (instrumentation, methods)
  • Genetic Vectors (genetics, metabolism)
  • Humans
  • Intestinal Diseases (genetics, immunology, therapy)
  • Intestinal Mucosa (immunology, microbiology)
  • Mice

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