Abstract | CONTEXT:
Ascorbic acid (AA) is a naturally occurring organic compound with antioxidant properties. It is necessary for normal growth and development, and has been shown to protect against tissue toxicity and oxidative stress. OBJECTIVE: MATERIALS AND METHODS: Male albino rats of Wistar strain (4-6 weeks old) weighing 125-150 g were used in this study. Animals were given a single dose of Fe-NTA (9 mg/kg body weight, intraperitoneal) after a week of treatment with AA (1 and 2 mg/animal/day). RESULTS:
Fe-NTA treatment enhanced microsomal lipid peroxidation (LPO) and hydrogen peroxide (H2O2) generation to 1.7- to 2.2-fold, glutathione (GSH) levels were decreased by two-fold and the activities of GSH metabolizing enzymes decreased to a range of 2.2- to 2.5-fold in renal tissue. These changes were reversed significantly in animals receiving pretreatment of AA. Treatment of rats with AA prior to the treatment with Fe-NTA decreased microsomal LPO and H2O2 generation to 124 and 172%, and also resulted in the recovery of reduced levels of GSH, GSH-metabolizing enzymes to almost 92% at the higher dose level of AA. DISCUSSION AND CONCLUSION: AA protects against Fe-NTA-induced nephrotoxicity and renal damage. AA has a beneficial impact on Fe-NTA-induced toxicity due to its scavenging and antioxidant effect in albino rats.
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Authors | S Ansar, M Iqbal |
Journal | Pharmaceutical biology
(Pharm Biol)
Vol. 51
Issue 12
Pg. 1559-63
(Dec 2013)
ISSN: 1744-5116 [Electronic] England |
PMID | 24024952
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antioxidants
- Ferric Compounds
- Glutathione Peroxidase
- Glutathione Transferase
- Glutathione
- Nitrilotriacetic Acid
- Ascorbic Acid
- ferric nitrilotriacetate
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Topics |
- Animals
- Antioxidants
(therapeutic use)
- Ascorbic Acid
(therapeutic use)
- Ferric Compounds
(toxicity)
- Glutathione
(metabolism)
- Glutathione Peroxidase
(metabolism)
- Glutathione Transferase
(metabolism)
- Kidney Diseases
(chemically induced, metabolism, prevention & control)
- Lipid Peroxidation
(drug effects)
- Male
- Microsomes
(drug effects, metabolism)
- Nitrilotriacetic Acid
(analogs & derivatives, toxicity)
- Oxidative Stress
(drug effects)
- Rats
- Rats, Wistar
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