Abstract |
In this study, we prepared solid lipid nanoparticles (TV-SLNs) loaded with toad venom extract and investigated their anti- tumor effects in vitro in HeLa and SKOV-3 cells. TV-SLNs were prepared using a cold homogenization technique, and the formulation was optimized by central composite design and response surface methods. The anti- tumor activities of TV-SLNs were evaluated by analyzing cell division and cell cycle distribution by using the MTT assay and flow cytometry. After incubation with TV-SLNs, the growth of both HeLa and SKOV-3 cells was inhibited significantly. The percentage of HeLa cells in G0/G1 phase decreased, whereas that in the S and G2/M phases increased. Thus, the S and G2/M phases were blocked after the incubation of HeLa cells with TV-SLNs for 24 h. In contrast, the percentage of SKOV-3 cells in G0/G1 phase increased and then decreased in S and G2/M phases, with the G0/G1 phase being blocked after incubation with TV-SLNs for 24 h. Our results demonstrate that TV-SLNs inhibited the fissiparism of HeLa and SKOV-3 cells in a time-and dose-dependent manner. TV-SLNs may be effective as a novel TV vaginal delivery system for the treatment of cervical and ovarian cancers.
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Authors | Su-Juan Zhang, Yong-Tai Zhang, Ji-Hui Zhao, Li-Na Shen, Feng Shi, Nian-Ping Feng |
Journal | Die Pharmazie
(Pharmazie)
Vol. 68
Issue 8
Pg. 653-60
(Aug 2013)
ISSN: 0031-7144 [Print] Germany |
PMID | 24020119
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Amphibian Venoms
- Antineoplastic Agents
- Suspensions
- Tetrazolium Salts
- Thiazoles
- thiazolyl blue
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Topics |
- Administration, Intravaginal
- Amphibian Venoms
(administration & dosage, chemistry, pharmacology)
- Antineoplastic Agents
(administration & dosage, chemistry, pharmacology)
- Cell Cycle
(drug effects)
- Chemistry, Pharmaceutical
- Chromatography, High Pressure Liquid
- Drug Compounding
- Drug Delivery Systems
- Drug Design
- Female
- HeLa Cells
- Humans
- Microscopy, Electron, Transmission
- Nanoparticles
- Ovarian Neoplasms
(drug therapy)
- Particle Size
- Suspensions
- Tetrazolium Salts
- Thiazoles
- Uterine Cervical Neoplasms
(drug therapy)
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