HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Preparation and in vitro anti-tumor properties of toad venom extract-loaded solid lipid nanoparticles.

Abstract
In this study, we prepared solid lipid nanoparticles (TV-SLNs) loaded with toad venom extract and investigated their anti-tumor effects in vitro in HeLa and SKOV-3 cells. TV-SLNs were prepared using a cold homogenization technique, and the formulation was optimized by central composite design and response surface methods. The anti-tumor activities of TV-SLNs were evaluated by analyzing cell division and cell cycle distribution by using the MTT assay and flow cytometry. After incubation with TV-SLNs, the growth of both HeLa and SKOV-3 cells was inhibited significantly. The percentage of HeLa cells in G0/G1 phase decreased, whereas that in the S and G2/M phases increased. Thus, the S and G2/M phases were blocked after the incubation of HeLa cells with TV-SLNs for 24 h. In contrast, the percentage of SKOV-3 cells in G0/G1 phase increased and then decreased in S and G2/M phases, with the G0/G1 phase being blocked after incubation with TV-SLNs for 24 h. Our results demonstrate that TV-SLNs inhibited the fissiparism of HeLa and SKOV-3 cells in a time-and dose-dependent manner. TV-SLNs may be effective as a novel TV vaginal delivery system for the treatment of cervical and ovarian cancers.
AuthorsSu-Juan Zhang, Yong-Tai Zhang, Ji-Hui Zhao, Li-Na Shen, Feng Shi, Nian-Ping Feng
JournalDie Pharmazie (Pharmazie) Vol. 68 Issue 8 Pg. 653-60 (Aug 2013) ISSN: 0031-7144 [Print] Germany
PMID24020119 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Amphibian Venoms
  • Antineoplastic Agents
  • Suspensions
  • Tetrazolium Salts
  • Thiazoles
  • thiazolyl blue
Topics
  • Administration, Intravaginal
  • Amphibian Venoms (administration & dosage, chemistry, pharmacology)
  • Antineoplastic Agents (administration & dosage, chemistry, pharmacology)
  • Cell Cycle (drug effects)
  • Chemistry, Pharmaceutical
  • Chromatography, High Pressure Liquid
  • Drug Compounding
  • Drug Delivery Systems
  • Drug Design
  • Female
  • HeLa Cells
  • Humans
  • Microscopy, Electron, Transmission
  • Nanoparticles
  • Ovarian Neoplasms (drug therapy)
  • Particle Size
  • Suspensions
  • Tetrazolium Salts
  • Thiazoles
  • Uterine Cervical Neoplasms (drug therapy)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: