Abstract |
Guinea pig gallbladder bile contains chenodeoxycholic acid (62 +/- 5%), ursodeoxycholic acid (8 +/- 5%), and 7-ketolithocholic acid (30 +/- 5%). All three bile acids became labeled to the same specific activity within 30 min after [3H] cholesterol was injected into bile fistula guinea pigs. When a mixture of [3H] ursodeoxycholic acid and [14C] chenodeoxycholic acid was infused into another bile fistula guinea pig, little 3H could be detected in either chenodeoxycholic acid or 7-ketolithocholic acid. But, 14C was efficiently incorporated into ursodeoxycholic and 7-ketolithocholic acids. Monohydroxylated bile acids make up 51% and ursodeoxycholic acid 38% of fecal bile acids. After 3 weeks of antibiotic therapy, lithocholic acid was reduced to 6% of the total, but ursodeoxycholic acid (5-11%) and 7-ketolithocholic (15-21%) acid persisted in bile. Lathosterol constituted 19% of skin sterols and was detected in the feces of an antibiotic-fed animal. After one bile fistula guinea pig suffered a partial biliary obstruction, ursodeoxycholic and 7-ketolithocholic acids increased to 46% and 22% of total bile acids, respectively. These results demonstrate that chenodeoxycholic acid, ursodeoxycholic acid, and 7-ketolithocholic acid can all be made in the liver of the guinea pig.
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Authors | G S Tint, G R Xu, A K Batta, S Shefer, W Niemann, G Salen |
Journal | Journal of lipid research
(J Lipid Res)
Vol. 31
Issue 7
Pg. 1301-6
(Jul 1990)
ISSN: 0022-2275 [Print] United States |
PMID | 2401860
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Bile Acids and Salts
- Deoxycholic Acid
- Chenodeoxycholic Acid
- 7-ketolithocholic acid
- Lithocholic Acid
- Ursodeoxycholic Acid
- lathosterol
- Cholesterol
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Topics |
- Animals
- Bile Acids and Salts
(analysis, metabolism)
- Chenodeoxycholic Acid
(analysis, metabolism)
- Cholesterol
(analysis)
- Deoxycholic Acid
(analogs & derivatives)
- Feces
(analysis)
- Guinea Pigs
- Isomerism
- Kinetics
- Lithocholic Acid
(analogs & derivatives, analysis, metabolism)
- Male
- Ursodeoxycholic Acid
(analysis, metabolism)
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