Enhanced binding to and killing of hepatocellular carcinoma cells in vitro by melittin when linked with a novel targeting peptide screened from phage display.
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| Abstract |
A random phage 12-mer peptide library and a whole-cell subtractive biopanning protocol against HepG2 cells were used to select a novel peptide-specific binding to hepatocellular carcinoma cells. As a result, peptide SLSLITMLKISR (AM-2) was screened as a novel homing peptide to hepatocellular carcinoma cells, tested by immunofluorescence and immunochemistry assays. Subsequently, peptide AM-2 was linked to melittin by A(EAAAK)2A, and the antitumor effect of this ligation product was detected by MTT assay, fluorescence-activated cell sorting, and scanning electron microscopy methods. Results of cell growth inhibition tests confirmed that the affinity of melittin was increased after being incorporated into AM-2, and AM-2-melittin specifically targeted and killed HepG2 cells in vitro. Thus, AM-2 is a valuable ligand for tumor targeting, which leads to increased binding and killing effect of hepatocellular carcinoma cells in vitro when ligated to melittin, and AM-2-melittin has a clinical potential application as target agents for the treatment of human hepatocellular carcinoma.
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| Authors | Honglei Zhao, Xin Feng, Wenyu Han, Yuwen Diao, Dong Han, Xiaofeng Tian, Yu Gao, Shanshan Liu, Seng Zhu, Cuimei Yao, Jingmin Gu, Changjiang Sun, Liancheng Lei |
| Journal | Journal of peptide science : an official publication of the European Peptide Society (J Pept Sci) Vol. 19 Issue 10 Pg. 639-50 (Oct 2013) ISSN: 1099-1387 [Electronic] England |
| PMID | 24014474 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2013 European Peptide Society and John Wiley & Sons, Ltd. |
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