Abstract |
Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) is a scaffold protein known to interact with a number of cancer-related proteins. nherf1 Mutations (K172N and D301V) were recently identified in breast cancer cells. To investigate the functional properties of NHERF1, wild-type and cancer-derived nherf1 mutations were stably expressed in SKMES-1 cells respectively. NHERF1-wt overexpression suppressed the cellular malignant phenotypes, including proliferation, migration, and invasion. nherf1 Mutations (K172N and D301V) caused complete or partial loss of NHERF1 functions by affecting the PTEN/NHERF1/PDGFRβ complex formation, inactivating NHERF1 inhibition of PDGF-induced AKT and ERK activation, and attenuating the tumor-suppressor effects of NHERF1-wt. These results further demonstrated the functional consequences of breast cancer-derived nherf1 mutations (K172N and D301V), and suggested the causal role of NHERF1 in tumor development and progression.
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Authors | Shan Cheng, Yang Li, Ying Yang, Duiping Feng, Longyan Yang, Qian Ma, Shuai Zheng, Ran Meng, Shuhui Wang, Songlin Wang, Wen G Jiang, Junqi He |
Journal | FEBS letters
(FEBS Lett)
Vol. 587
Issue 20
Pg. 3289-95
(Oct 11 2013)
ISSN: 1873-3468 [Electronic] England |
PMID | 24012959
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Federation of European Biochemical Societies. All rights reserved. |
Chemical References |
- Phosphoproteins
- Sodium-Hydrogen Exchangers
- sodium-hydrogen exchanger regulatory factor
- Receptor, Platelet-Derived Growth Factor beta
- PTEN Phosphohydrolase
- PTEN protein, human
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Topics |
- Animals
- Breast Neoplasms
(genetics, metabolism)
- COS Cells
- Cell Line, Tumor
- Cell Proliferation
- Chlorocebus aethiops
- Female
- Humans
- Mutation
- PTEN Phosphohydrolase
(genetics, metabolism)
- Phosphoproteins
(genetics, metabolism)
- Protein Binding
- Receptor, Platelet-Derived Growth Factor beta
(genetics, metabolism)
- Sodium-Hydrogen Exchangers
(genetics, metabolism)
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